Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts
Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts
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DOI：
10.1016/j.tiv.2008.12.021
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发表时间：
2009-04-01
影响因子：
3.2
通讯作者：
Ulrichova, J.
中科院分区：
文献类型：
--
作者：
Zdarilova, A.;Svobodova, A.;Ulrichova, J.
牙周炎是一种与牙齿支持组织炎症相关的慢性疾病。炎症是由一组革兰氏阴性厌氧菌引起的。这些表达了许多刺激因子，包括脂多糖（LPS），它在牙周病的发展中起着关键作用。具有抗炎和抗微生物特性的植物提取物已被证明可以抑制菌斑的形成，从而预防慢性牙龈炎。本实验研究了夏枯草提取物对小鼠的抗氧化作用。提取物（PVE; 5、10、25 μ g/ml）及其组分迷迭香酸（RA; 1 μ g/ml）对人牙龈成纤维细胞中LPS诱导的氧化损伤和炎症的作用。PVE和RA减少活性氧（ROS）的产生，细胞内谷胱甘肽（GSH）消耗以及脂质过氧化作用在LPS处理的细胞。PVE和RA治疗也抑制LPS诱导的白细胞介素1 β（IL-10）的上调。白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）和诱导型一氧化氮合酶（iNOS）的抑制表达。结果表明，PVE和RA能够抑制LPS诱导的牙龈成纤维细胞的生物学变化。PVE和RA的作用可能与其抗炎活性有关，因此PVE和RA的使用可能与调节炎症过程（包括牙周病）有关。(c)2009爱思唯尔有限公司版权所有。
Periodontitis is a chronic disease associated with inflammation of the tooth-supporting tissues. The inflammation is initiated by a group of gram-negative anaerobic bacteria. These express a number of irritating factors including a lipopolysaccharide (LPS), which plays a key role in periodontal disease development. Plant extracts with anti-inflammatory and anti-microbial properties have been shown to inhibit bacterial plaque formation and thus prevent chronic gingivitis. In this study we tested effects of Prunella vulgaris L. extract (PVE; 5, 10, 25 mu g/ml) and its component rosmarinic acid (RA; 1 mu g/ml) on LPS-induced oxidative damage and inflammation in human gingival fibroblasts. PVE and RA reduced reactive oxygen species (ROS) production, intracellular glutathione (GSH) depletion as well as lipid peroxidation in LPS-treated cells. Treatment with PVE and RA also inhibited LPS-induced up-regulation of interleukin 1 beta (IL-10). interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and suppressed expression of inducible nitric oxide synthase (iNOS). The results indicate that PVE and RA are able to suppress LPS-induced biological changes in gingival fibroblasts. The effects of PVE and RA are presumably linked to their anti-inflammatory activities and thus use of PVE and RA may be relevant in modulating the inflammation process, including periodontal disease. (c) 2009 Elsevier Ltd. All rights reserved.