A STANDARDIZED METHOD FOR IDENTIFICATION OF LICHEN PRODUCTS
A STANDARDIZED METHOD FOR IDENTIFICATION OF LICHEN PRODUCTS
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DOI：
10.1016/s0021-9673(00)83967-9
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
KRISTINSSON, HD
中科院分区：
文献类型：
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作者：
CULBERSON, CF;KRISTINSSON, HD
本文介绍了一种用薄层层析法对地衣真菌产物进行常规鉴定的方法。植物碎片的微萃取物在三种溶剂系统中进行了层析。未知的斑点被分配到由每个平板上层析的两种地衣物质(青蒿素和降冰片酸)的标记对照的RF值定义的RF类。未知数仍然是通过分类(按RF类)打孔的卡片来识别的，卡片汇总了之前研究的所有化合物的微量化学数据。然后，通过额外的微量化学测试来确认初步鉴定。开放式系统可以合并新的和未知的化合物以及来自其他色谱系统的信息。给出了104种产品的标准化程序所获得的数据。
A procedure for the routine identification of the products of lichen-forming fungi by thin-layer chromatography is described. Microextracts of plant fragments are chromatographed in three solvent systems. The spots of unknowns are assigned toRFclasses defined by theRFvalues of marker controls of two lichen substances (atranorin and norstictic acid) chromatographed on every plate. The unknowns are tenatively identified by sorting (byRFclasses) punched cards summarizing microchemical data for all compounds previously studied. The preliminary identification is then confirmed by additional microchemical tests. The open-ended system can incorporate new and unknown compounds as well as information from other chromatographic systems. Data obtained by the standardized procedure are given for 104 products.