Expression level of lipoprotein lipase and dystrophin genes predict survival in B-cell chronic lymphocytic leukemia
Expression level of lipoprotein lipase and dystrophin genes predict survival in B-cell chronic lymphocytic leukemia
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DOI：
10.1080/10428190701245112
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Vorobjev, A. I.
中科院分区：
文献类型：
--
作者：
Nikitin, E. A.;Malakho, S. G.;Vorobjev, A. I.
免疫球蛋白可变区基因（vh基因）的突变状态被认为是B-CLL长期预后的最强预测因子。然而，在常规临床实践中的应用是耗时的，因此需要一些其他的预测。在这项研究中，我们比较了实时PCR定量检测在vh未突变和突变B-CLL亚型中差异表达的四种基因的预后价值：ZAP-70、ZBTB20、DMD和LPL。该研究包括134名B-CLL患者。LPL和DMD基因的表达水平与突变状态显著相关，而ZAP-70基因的表达水平仅在CD19+选择病例中相关（N = 40）。ZBTB20基因无相关性。LPL和DMD的表达水平预测了整个队列患者的总生存期。LPL基因表达水平的预后价值即使在a期CLL患者中也是显著的。用于测量LPL基因表达的定量RT-PCR检测足够强大，可以引入常规临床实践。
Mutational status of immunoglobulin variable region genes ( VH-genes) is known as the strongest predictor of long term prognosis in B-CLL. However, applications in the routine clinical practice are time consuming, and therefore some other predictions are required. In this study, we have compared prognostic values of real time PCR quantification of the expression levels of four genes previously shown to be differentially expressed in VH-unmutated and mutated B-CLL subtypes: ZAP-70, ZBTB20, DMD and LPL. The study included 134 B-CLL patients. Expression levels of LPL and DMD genes were significantly correlated to mutational status, while expression levels of of ZAP-70 gene correlated only in CD19+ selected cases ( N = 40). No correlation was observed for ZBTB20 gene. Expression levels of LPL and DMD predicted overall survival in the entire cohort of patients. Prognostic values of LPL gene expression levels were significant even for CLL patients with stage A. Quantitative RT-PCR assays for measuring LPL gene expression are robust enough to be introduced into routine clinical practice.