Effects of altrenogest and exogenous gonadotropins on ovarian function and embryo recovery in swine leukocyte antigen inbred miniature swine as influenced by cystic endometrial hyperplasia.
Effects of altrenogest and exogenous gonadotropins on ovarian function and embryo recovery in swine leukocyte antigen inbred miniature swine as influenced by cystic endometrial hyperplasia.
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受囊性子宫内膜增生影响的双体孕素和外源性促性腺激素对猪白细胞抗原近交小型猪卵巢功能和胚胎恢复的影响。
DOI：
10.1095/biolreprod35.5.1261
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发表时间：
1986
影响因子：
3.6
通讯作者：
D. E. Wildt
中科院分区：
文献类型：
--
作者：
John R. Diehl;John R. Diehl;L. Stuart;L. Stuart;K. Goodrowe;K. Goodrowe;K. Goodrowe;D. E. Wildt;D. E. Wildt
将合成孕激素烯丙孕素（ALT）和外源性促性腺激素给予猪白细胞抗原（SLA）近交系小型猪，该小型猪通常具有降低的排卵率和小的产仔数。在研究1中，15只成年雌性猪为对照组（组I，n = 5），饲喂15 mg ALT/天，持续14天（组II，n = 5），或饲喂ALT并用孕马血清促性腺激素（1200 IU，i.m.）和人绒毛膜促性腺激素（500 IU，i.m.）分别在ALT停药后24 h和104 h（组III，n = 5）。在研究2中，三组猪（I，n = 24; II，n = 11; III，n = 11）的处理与研究1相似，但研究2中的猪（组II和III）交配。在发情后5-8天，通过腹腔镜或剖腹手术检查两项研究中所有动物的卵巢，以评估卵巢活性。研究2中第II组和第III组动物的子宫用组织培养基冲洗，评估胚胎的存在和质量。在ALT给药期间，没有猪表现出发情行为。ALT停药后，并非所有动物均表现出典型的发情行为。当合并两项研究的数据时，组II中2/16头猪（12.5%）和组III中6/16头猪（37.5%）未能显示站立发情。ALT停药后，第II组和第III组雌性动物分别在5.6 +/- 0.3天和5.8 +/- 0.3天恢复发情（p> 0.05）。（250字处删节）
Altrenogest (ALT), a synthetic progestogen, and exogenous gonadotropins were administered to the Swine Leukocyte Antigen (SLA) inbred miniature pig that typically has a reduced ovulation rate and small litter size. In Study 1, 15 adult female pigs were either controls (Group I, n = 5), fed 15 mg ALT/day for 14 days (Group II, n = 5), or fed ALT and treated with pregnant mare's serum gonadotropin (1200 IU, i.m.) and human chorionic gonadotropin (500 IU, i.m.) 24 h and 104 h, respectively, after ALT withdrawal (Group III, n = 5). In Study 2, three groups of pigs (I, n = 24; II, n = 11; III, n = 11) were treated similarly to those in Study 1 except that pigs in Study 2 (Groups II and III) were mated. Ovaries of all animals in both studies were examined laparoscopically or by laparotomy 5-8 days after estrus to assess ovarian activity. The uteri of Group II and III animals in Study 2 were flushed with tissue culture medium, which was evaluated for the presence and quality of embryos. No pig demonstrated estrous behavior during ALT administration. Not all animals demonstrated typical estrous behavior after ALT withdrawal. When the data from both studies were combined, 2 of 16 (12.5%) of group II and 6 of 16 (37.5%) of Group III pigs failed to demonstrate standing estrus. Following ALT withdrawal, females in Groups II and III returned to estrus in 5.6 +/- 0.3 and 5.8 +/- 0.3 days, respectively (p greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)