Human papillomavirus infection requires cell surface heparan sulfate
Human papillomavirus infection requires cell surface heparan sulfate
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DOI：
10.1128/jvi.75.3.1565-1570.2001
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发表时间：
2001-02-01
影响因子：
5.4
通讯作者：
Sapp, M
中科院分区：
文献类型：
--
作者：
Giroglou, T;Florin, L;Sapp, M
通过对细胞系的假感染，我们证明了HPV-16和HPV-33假病毒粒子感染细胞表面是必需的。假性感染可被肝素抑制，但不能被皮肤素或硫酸软骨素抑制，通过降低表面硫酸盐化水平来减少，并通过肝素酶处理来消除。羧基末端缺失的HPV-33病毒样颗粒仍然有效地与肝素结合。抗血清或肝素中和后脱落的动力学表明，假病毒粒子在细胞表面从对肝素敏感的结合模式转变为对肝素抵抗的结合模式，可能涉及二级受体。α-6整合素不是HPV-33假感染的受体。
Using pseudoinfection of cell lines, we demonstrate that cell surface heparan sulfate is required for infection by human papillomavirus type 16 (HPV-16) and HPV-33 pseudovirions. Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by heparinase treatment. Carboxy-terminally deleted HPV-33 virus-like particles still bound efficiently to heparin. The kinetics of postattachment neutralization by antiserum or heparin indicated that pseudovirions were shifted on the cell surface from a heparin sensitive into a heparin-resistant mode of binding, possibly involving a secondary receptor. Alpha-6 integrin is not a receptor for HPV-33 pseudoinfection.