Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells.
Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells.
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DOI：
10.1038/celldisc.2016.18
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发表时间：
2016
期刊：
影响因子：
33.5
通讯作者：
Friml J
中科院分区：
文献类型：
--
作者：
Łangowski Ł;Wabnik K;Li H;Vanneste S;Naramoto S;Tanaka H;Friml J
真核细胞质膜结构域中蛋白质的不对称定位是细胞极性的基本表现，是多细胞组织和发育模式的核心。在植物中，货物蛋白的极性定位的机制仍然是未知的，似乎是从根本上不同于哺乳动物中的运作。在这里，我们提出了一个系统的，定量的比较分析的极性传递和亚细胞定位的蛋白质，其特征在于不同的极性质膜结构域在植物细胞中。显微镜分析和计算建模的结合揭示了一个机制框架常见的不同的极性货物和潜在的建立和维护顶端，基底和侧极域在植物细胞。这一机制依赖于极性分泌、组成性内吞再循环和质膜内物质受限的侧向扩散。此外，我们的观察表明，极性货物分布涉及单个蛋白质在质膜内形成簇并与细胞外基质相互作用的潜力。我们的观察提供了深入了解极性货物交付和极性维持在植物细胞中的共享细胞机制。
The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells.