Quantification of trace elements by sector field inductively coupled plasma mass spectrometry in urine, serum, blood and cerebrospinal fluid of patients with Parkinson's disease
Quantification of trace elements by sector field inductively coupled plasma mass spectrometry in urine, serum, blood and cerebrospinal fluid of patients with Parkinson's disease
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DOI：
10.1016/j.sab.2004.02.007
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发表时间：
2004-04-30
影响因子：
3.3
通讯作者：
Senofonte, O
中科院分区：
文献类型：
--
作者：
Bocca, B;Alimonti, A;Senofonte, O
为探讨帕金森病（PD）患者体内微量金属及氧化物的水平是否与PD有关，测定了26例PD患者和13例正常对照者的尿、血清、血液和脑脊液（CSF）中At、Be、Cd、Co、Cr、Ha、Mn、Ni、Pb和V的含量，并测定了血清过氧化物和抗氧化能力。脑脊液、血清和尿液经1 + 4水稀释，血液经常压酸辅助微波消解，扇形场电感耦合等离子体质谱（SF-ICP-MS）测定。结果表明，铅和钒在血液和尿液中的浓度显着增加（P小于或等于0.03，在这两种情况下）有关的疾病。帕金森病似乎也与血清中Al、Cd、Hg和Pb以及CSF中Cd、Co、Cr、Hg和Pb的水平降低密切相关（P <0.003）。至于Mn，在PD患者的CSF和全血中发现的平均浓度低于对照组，尽管这种趋势在统计学上不显著。与对照组相比，PD患者的过氧化物水平也增加（P小于或等于0.001），而抗氧化能力较低（P小于或等于0.002）。(C)2004年由Elsevier B. V.出版
To assess whether levels of trace metals and oxidative species are involved in Parkinson's disease (PD), At, Be, Cd, Co, Cr, Ha, Mn, Ni, Pb and V were measured in urine, serum, blood and cerebrospinal fluid (CSF) and serum peroxides and antioxidant capacity were determined in 26 patients with PD and 13 control subjects. The quantification of metals was based on the 1 + 4 water dilution of CSF, serum and urine, the acid-assisted microwave digestion under atmospheric pressure of blood and final determination by sector field inductively coupled plasma mass spectrometry (SF-ICP-MS). Results indicated a significant increase of Pb and V concentrations in blood and urine (P less than or equal to 0.03, in both cases) related to the disease. Parkinson disease also seemed to be closely associated (P less than or equal to 0.003) with a reduction in levels of Al, Cd, Hg and Pb in serum and of Cd, Co, Cr, Hg, Pb in CSF As regards Mn, a lower mean concentration was found in the CSF and whole blood of PD patients than in control group, although this trend was not statistically significant. Levels of peroxides were also increased (P less than or equal to 0.001), while antioxidant capacity was lower (P less than or equal to 0.002) in PD patients than in controls. (C) 2004 Published by Elsevier B.V.