Cancer/Testis Antigens and Clinical Risk Factors for Liver Metastasis of Colorectal Cancer: A Predictive Panel
Cancer/Testis Antigens and Clinical Risk Factors for Liver Metastasis of Colorectal Cancer: A Predictive Panel
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癌症/睾丸抗原和结直肠癌肝转移的临床危险因素：预测面板
DOI：
10.1007/dcr.0b013e3181bdca3a
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发表时间：
2010-01-01
影响因子：
3.9
通讯作者：
Gu, Jin
中科院分区：
文献类型：
--
作者：
Chen, ZhiGuo;Li, Ming;Gu, Jin
目的：越来越多的证据表明，癌/睾丸抗原可能作为肿瘤恶性表型的指标。本研究的目的是评估癌/睾丸抗原基因在预测结直肠癌向肝脏转移中的作用。方法：采用反转录聚合酶链反应法检测288例结直肠癌原发肿瘤或肝转移组织标本中25个癌/睾丸抗原基因的表达水平。采用Pearson chi(2)和多元logistic回归分析评估危险因素与结直肠癌肝转移概率的关系。结果：结直肠癌组织样本中癌/睾丸抗原基因的表达模式在原发肿瘤和肝转移瘤中无显著差异。而3个癌/睾丸抗原基因（PAGE4、SCP-1、SPANX）和3个临床病理参数（淋巴结累及、血管癌栓塞、肿瘤浸润深度）与结直肠癌肝转移有显著相关性（P < 0.05）。我们建立了一个逻辑回归模型来预测肝转移，该模型基于PAGE4、淋巴结受累和血管癌栓塞的存在与否组成的面板。基于该小组预测的肝转移风险与实际观察到的风险一致。当所有3个因素均为阳性时，专家组估计发生肝转移的概率为86.9%，与淋巴结累及和血管癌栓塞的经典方法相比，预测水平提高了20%。结论：一种新的包括PAGE4表达的预测面板可能有助于预测结直肠癌的肝转移。
PURPOSE: Accumulating evidence suggests that cancer/testis antigens may serve as indicators of tumor malignant phenotype. The purpose of this study is to evaluate cancer/testis antigen genes in predicting metastasis of colorectal cancer to the liver.METHODS: The expression levels of 25 cancer/testis antigen genes were determined by reverse-transcription polymerase chain reaction in 288 colorectal cancer tissue samples from the primary tumor or liver metastasis. Pearson chi(2) and multiple logistic regression analyses were performed to assess the association between risk factors and probability of liver metastasis of colorectal cancer.RESULTS: No significant difference was detected between the primary tumor and liver metastasis in expression pattern of cancer/testis antigen genes in colorectal cancer tissue samples. However, 3 cancer/testis antigen genes (PAGE4, SCP-1, and SPANX) and 3 clinicopathologic parameters (lymph node involvement, vessel cancer embolus, and tumor invasion depth) correlated significantly with liver metastasis of colorectal cancer (P < .05). A logistic regression model was constructed for prediction of liver metastasis based on a panel consisting of PAGE4, lymph node involvement, and presence or absence of vessel cancer embolus. The predicted risk of liver metastasis based on the panel was consistent with the actual risk observed. The probability of developing liver metastasis as estimated by the panel was 86.9% when all 3 factors were positive, representing an up to 20% improvement in the prediction level compared with the classic methods of lymph node involvement and vessel cancer embolus.CONCLUSIONS: A new predictive panel including PAGE4 expression may help predict liver metastasis of colorectal cancer.