Increased presence of cells with multiple elongated processes in osteoarthritic femoral head cartilage
Increased presence of cells with multiple elongated processes in osteoarthritic femoral head cartilage
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DOI：
10.1016/j.joca.2003.09.001
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发表时间：
2004-01-01
影响因子：
7
通讯作者：
Knight, MM
中科院分区：
文献类型：
--
作者：
Holloway, I;Kayser, M;Knight, MM
目的：本研究探讨了骨关节炎（OA）和非OA人类股骨头关节软骨中的软骨细胞的形态，特别是一个亚群的细胞与多个细长的过程辐射到30 μ m的细胞外matrix.Methods：软骨外植体的外观从8个解剖部位的OA（n=6）和非OA（n=5）股骨头的表面。标记细胞的波形蛋白中间丝，并使用落射荧光和共聚焦显微镜观察。与细长的过程中的细胞的百分比与宏观和组织学指标osteoarthritis.Results：细胞与过程占不到10%的总细胞群在非OA软骨。相比之下，在CA股骨头的外周区域中，这些细胞占总细胞群的20-45%，差异具有统计学意义。这些外周区域通常不承重，也最有可能显示严重纤颤和血管翳形成。一个统计学上显着的相关性之间的百分比与过程的细胞和OA降解的组织学程度，量化的Mankin score.Conclusions：细胞过程，这可能是与本地化的pericellular基质的分解的延伸，无疑会改变许多方面的细胞功能，包括表型表达和mechanotransduction。因此，软骨细胞形态的这些显著变化可能对骨关节炎的病因学和潜在治疗策略的发展具有重要意义。(C)2003年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: This study examined the morphology of chondrocytes in articular cartilage from osteoarthritic (OA) and non-OA human femoral heads and in particular the appearance of a sub-population of cells with multiple elongated processes radiating up to 30 mum into the extracellular matrix.Methods: Cartilage explants were removed from 8 anatomical sites over the surface of OA (n=6) and non-OA (n=5) femoral heads. Cells were labeled for vimentin intermediate filaments and visualized using epi-fluorescence and confocal microscopy. The percentage of cells with elongated processes was correlated with macroscopic and histological indicators of osteoarthritis.Results: Cells with processes accounted for less than 10% of the total cell population in non-OA cartilage. By contrast, in the peripheral regions of the CA femoral head these cells accounted for 20-45% of the total cell population, the differences being statistically significant. These peripheral areas are habitually non-load bearing and were also the most likely to show gross fibrillation and pannus formation. A statistically significant correlation was demonstrated between the percentage of cells with processes and the histological extent of the OA degradation, quantified in terms of the Mankin score.Conclusions: The extension of cell processes, which may be associated with localized breakdown of the pericellular matrix, will undoubtedly alter numerous aspects of cell function including phenotypic expression and mechanotransduction. Hence these significant changes in chondrocyte morphology are likely to have important implications for the aetiology of osteoarthritis and the development of potential treatment strategies. (C) 2003 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.