Effect of tannic acid on the development and resistance of the Gypsy moth Lymantria dispar L. to viral infection
Effect of tannic acid on the development and resistance of the Gypsy moth Lymantria dispar L. to viral infection
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DOI：
10.1134/s1607672906040077
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发表时间：
2006-07
影响因子：
0.8
通讯作者：
V. Martemyanov;S. Bakhvalov;I. Dubovskiy;V. Glupov;N. Salakhutdinov;G. Tolstikov
中科院分区：
文献类型：
--
作者：
V. Martemyanov;S. Bakhvalov;I. Dubovskiy;V. Glupov;N. Salakhutdinov;G. Tolstikov
材料与方法以舞毒蛾幼虫为研究对象。这些昆虫是在自然种群的卵期采集的，然后在实验室条件下生长。实验在春季和夏季进行。这些昆虫要么生长在培养皿中的人工营养介质中，要么生长在23-25℃、每天16小时光照周期下的饲养笼中。饲料用0.9%单宁酸水溶液处理(Sigma，美国)。这个浓度与前一年落叶的桦树叶中单宁的含量相对应[7]。用107多角体/毫升的病毒悬浮液对3龄幼虫进行细微分散喷洒，经口感染幼虫。幼虫的体重由群体称重决定，性别由蛹的形态特征--最后腹节的结构决定[2]。幼虫死亡率用整个幼虫期内死亡毛虫的总数百分比表示。用光学显微镜检测到死毛虫体内存在病毒多角体。
MATERIALS AND METHODSThis study was performed with the gypsy moth (L. dispar L., Lepidoptera: Lymantridae) caterpillars. The insects were collected at the egg phase in the natural population and then grown under laboratory conditions. The experiments were performed in spring and summer. The insects were grown either in an artificial nutrient medium in Petri dishes or on birch shoots in rearing cages at 23–25 C under at 16-h light period per day.Forage was treated with 0.9% aqueous solution of tannic acid (Sigma, United States). This concentration corresponds to the content of tannins in birch leaves defoliated in the previous year [7]. Caterpillars of the third instar were infected perorally by finely-dispersated spraying of forage with a viral suspension at a concentration of 107 polyhedrons/ml. Weight of caterpillars was determined by group weighing, and sex was determined by morphological traits of pupae—the structure of the last belly segments [2]. The mortality rate of the insects was expressed as the aggregate percentage of dead caterpillars over the entire period of larval stage. The presence of viral polyhedrons in the body of dead caterpillars was detected with a light microscope.