A Legionella pneumophila collagen-like protein encoded by a gene with a variable number of tandem repeats is involved in the adherence and invasion of host cells
A Legionella pneumophila collagen-like protein encoded by a gene with a variable number of tandem repeats is involved in the adherence and invasion of host cells
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DOI：
10.1111/j.1574-6968.2010.01951.x
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发表时间：
2010-05-01
影响因子：
2.1
通讯作者：
Anne, Jozef
中科院分区：
文献类型：
--
作者：
Vandersmissen, Liesbeth;De Buck, Emmy;Anne, Jozef
嗜肺军团菌是革兰氏阴性的兼性细胞内病原体，是军团病的病原体，军团病是一种严重的人类肺炎。军团菌基因组测序分析显示，该基因具有可变数量的串联重复序列（VNTRs），其数量因菌株而异。我们在108个临床、环境和温泉血清I型菌株中检测了该基因的菌株分布。鉴定出12个变异，但重复单位数量与临床和环境菌株之间没有相关性。编码的蛋白含有外膜蛋白的c端一致基序，并具有由VNTR区编码的大区域胶原样重复序列。因此，我们将该蛋白标记为Lcl，即军团菌胶原样蛋白。研究表明，Lcl有助于宿主细胞的粘附和侵袭，并且Lcl中存在的重复单元的数量对这些粘附特性有影响。
Legionella pneumophila is a Gram-negative, facultative intracellular pathogen and the causative agent of Legionnaires' disease, a severe pneumonia in humans. Analysis of the Legionella sequenced genomes revealed a gene with a variable number of tandem repeats (VNTRs), whose number varies between strains. We examined the strain distribution of this gene among a collection of 108 clinical, environmental and hot spring serotype I strains. Twelve variants were identified, but no correlation was observed between the number of repeat units and clinical and environmental strains. The encoded protein contains the C-terminal consensus motif of outer membrane proteins and has a large region of collagen-like repeats that is encoded by the VNTR region. We have therefore annotated this protein Lcl for Legionella collagen-like protein. Lcl was shown to contribute to the adherence and invasion of host cells and it was demonstrated that the number of repeat units present in lcl had an influence on these adhesion characteristics.