Electron Microscopic and Biochemical Study of the Neutrophilic Granules from Leucocytes
Electron Microscopic and Biochemical Study of the Neutrophilic Granules from Leucocytes
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白细胞中性粒细胞颗粒的电子显微镜和生化研究
DOI：
10.1093/oxfordjournals.jmicro.a049514
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发表时间：
1966
期刊：
影响因子：
--
通讯作者：
Akio Teraoka
中科院分区：
文献类型：
--
作者：
Y. Shinagawa;Y. Shinagawa;C. Tanaka;Akio Teraoka
用无菌生理盐水腹腔注射法诱导家兔炎性渗出液，收集嗜中性白细胞。通过差速离心分离嗜中性粒细胞以及其他亚细胞级分。通过薄切片和负染色方法在电子显微镜下观察每个级分。嗜中性粒细胞部分由密度较低的颗粒组成，即。A颗粒大小均匀（0.3 μ m）。直径）和密度。很少观察到线粒体和其他细胞组分的污染。颗粒的界膜为双层膜结构。膜的内部通常填充有直径为300 μ m的微粒。嗜酸性颗粒中偶见片状结构。该馏分含有一种新的细胞色素，作者将其命名为细胞色素B（558，中性粒细胞）。另一方面，“线粒体”部分含有非常少量的线粒体，如通过电子显微镜和分光光度法所揭示的。这部分主要由致密的嗜中性粒细胞颗粒，B-颗粒，其中存在一个新的血红素蛋白提出了分光光度法，并提出了一个暂定的命名，血红素蛋白560（中性粒细胞）。
The neutrophilic leucocytes were collected from inflammatory exudates induced in rabbits by intraperitoneal injection of sterile saline. Neutrophilic granules as well as other subcellular fractions were separeted by differential centrifugation. Each fraction was observed in an electron microscope, by means of both thin sectioning and negative staining methods. The neutrophilic granule fraction which consisted of less dense granules, ie. A-granules, was almost homogeneous in size (0.3 μ. diameter) and density. Contamination of mitochondria and other cellular components was scarecely observed. The limitting membrane of the granule has double-membrane structure. Inside of the membrane was generally filled with microgranules of 300 Å in diameter. Occasionally, lamellar structuer was observed in the neutrophilic granules. This fraction contains a new cytochrome which was designated as cytochrome B (558, neutrophils) by the authors. On the other hand, the “mitochondrial” fraction contained very small amount of mitochondria as revealed by both electron microscopy and spectrophotometry. This fraction was mainly composed of the dense neutrophilic granules, B-granules, in which the presence -of a new haemoprotein was suggested spectrophoto-metrically, and a tentative nomenclature, haemoprotein 560 (neutrophils) was proposed for this haemoprotein.