Karyotyping human chromosomes by combinatorial multi-fluor FISH
Karyotyping human chromosomes by combinatorial multi-fluor FISH
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DOI：
10.1038/ng0496-368
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发表时间：
1996-04-01
期刊：
影响因子：
30.8
通讯作者：
Ward, DC
中科院分区：
文献类型：
--
作者：
Speicher, MR;Ballard, SG;Ward, DC
我们已经开发了落射荧光滤光片组和计算机软件，用于检测和区分同时杂交的27种不同的DNA探针。对于核型分析，将一组人染色体涂染探针（每个探针标记有不同的荧光组合）与从正常细胞、临床标本和肿瘤细胞系制备的中期染色体杂交。简单和复杂的染色体重排都可以被迅速而明确地检测到，许多复杂的染色体异常不能用常规的细胞遗传学显带技术来描述。我们的数据表明，多重荧光原位杂交（M-FISH）可能有广泛的临床应用和补充标准的细胞遗传学，特别是复杂核型的表征。
We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the move complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes.