Activated inflammatory T cells correlate with lesion size in human cutaneous leishmaniasis
Activated inflammatory T cells correlate with lesion size in human cutaneous leishmaniasis
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DOI：
10.1016/j.imlet.2005.06.004
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发表时间：
2005-11-15
影响因子：
4.4
通讯作者：
Gollob, KJ
中科院分区：
文献类型：
--
作者：
Antonelli, LRV;Dutra, WO;Gollob, KJ
利什曼病是一种重要的寄生虫病，影响全世界数百万人。在试图理解使用流式细胞术测定的免疫学测量的临床相关性时，确定了一组明确的人利什曼病患者的几种免疫表型，并与疾病的临床测量（黑山皮肤试验（MST）和病变面积）相关。分析表明，MST大小和离体最近活化的CD 4（+）T细胞的频率之间呈正相关。相比之下，最近活化的CD 8（+）T细胞的频率较高与MST大小较小相关。此外，观察到病变总面积与活化的CD 69（+）（离体）和CD 40 L（+）（与利什曼原虫可溶性抗原（SLA）培养）T淋巴细胞的频率之间呈正相关。最后，较大的病变也与SLA特异性炎性细胞因子（IFN-γ或TNF-α）产生淋巴细胞的频率较高相关。这些研究表明，免疫学标志物与人类利什曼病的临床指标相关，有助于更好地了解这种重要的寄生虫病的演变。(c)2005 Elsevier B. V.保留所有权利。
Leishmaniasis is an important parasitic disease affecting millions worldwide. In attempts to understand the clinical relevance of immunological measurements as determined using flow cytometry, several immunological phenotypes were determined for a group of well defined human leishmaniasis patients and correlated with clinical measurements of the disease (Montenegro skin test (MST) and lesion area). The analysis demonstrated a positive correlation between the MST size and the frequency of ex vivo recent activated CD4(+) T cells. In contrast, higher frequencies of recent activated CD8(+) T cells were correlated with a smaller MST size. Moreover, a positive correlation was observed between the lesion total area and the frequency of activated CD69(+) (ex vivo) and CD40L(+) (cultured with Leishmania soluble antigen (SLA)) T lymphocytes. Finally, larger lesions were also correlated with a higher frequency of SLA specific inflammatory cytokine (IFN-gamma or TNF-alpha) producing lymphocytes. These studies demonstrate that immunological markers are correlated with clinical indicators of human leishmaniasis and serve to better understand the evolution of this important parasitic disease. (c) 2005 Elsevier B.V. All rights reserved.