Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs
Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs
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DOI：
10.1093/jn/133.4.1158
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发表时间：
2003-04-01
影响因子：
4.2
通讯作者：
von der Weid, T
中科院分区：
文献类型：
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作者：
Benyacoub, J;Czarnecki- Maulden, GL;von der Weid, T
肠道菌群在宿主的一些生理功能中起着至关重要的作用，包括在生命的最初几个月肠道相关淋巴组织的成熟。口服益生菌乳酸菌（LAB）可以调节人类和一些实验动物的免疫系统。这种效应从未在狗身上进行过研究；因此，我们的目的是研究益生菌乳酸菌刺激幼犬免疫功能的能力。幼犬被分为两组，从断奶至1岁，分别饲喂对照饲粮和添加5 × 108菌落形成单位(cfu)/d益生菌屎肠球菌（SF68）的饲粮。在不同时间点采集狗的粪便和血液样本，测定粪便免疫球蛋白（Ig）A、循环IgG和IgA以及淋巴样细胞亚群比例。接种益生菌组的粪便IgA和犬瘟热病毒（CDV）疫苗特异性循环IgG和IgA均高于对照组。各组间CD4+和CD8+ T细胞百分比无差异，但成熟B细胞[CD21(+)/主要组织相容性复合体（MHC）类11(+)]的比例在益生菌喂养组中更高。这些数据首次表明，饲粮中添加益生菌乳酸菌可增强幼犬的特异性免疫功能，从而为益生菌在犬营养中的应用提供了新的机遇。
The gut microflora play a crucial role in several physiologic functions of the host, including maturation of the gut-associated lymphoid tissues during the first months of life. Oral administration of probiotic lactic acid bacteria (LAB) modulates the immune system of humans and some laboratory animals. This effect has never been examined in dogs; therefore, our aim was to study the capacity of a probiotic LAB to stimulate immune functions in young dogs. Puppies were allotted to two groups receiving either a control diet or a diet supplemented with 5 x 108 colony forming units (cfu)/d of probiotic Enterococcus faecium, (SF68) from weaning to 1 y. of age. Fecal and blood samples were collected from the dogs at different time points for the measurement of fecal immunoglobulin (Ig)A, circulating IgG and IgA, and the proportions of lymphoid cell subsets. Fecal IgA and canine distemper virus (CDV) vaccine-specific circulating IgG and IgA were higher in the group receiving the probiotic than in controls. There were no differences in the percentages of CD4+ and CD8+ T cells between the groups, but the proportion of mature B cells [CD21(+)/major histocompatibility complex (MHC) class 11(+)] was greater in those fed the probiotic. These data show for the first time that a dietary probiotic LAB enhance specific immune functions in young dogs, thus offering new opportunities for the utilization of probiotics in canine nutrition.