Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions
Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions
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DOI：
10.1093/emboj/cdg063
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发表时间：
2003-02-03
期刊：
影响因子：
11.4
通讯作者：
Schiebel, E
中科院分区：
文献类型：
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作者：
Maekawa, H;Usui, T;Schiebel, E
芽殖酵母纺锤体通过细胞质微管（CM）和细胞皮层之间的相互作用沿沿着母芽轴排列。Kar 9与EB 1相关蛋白Bim 1复合，介导CM与子细胞（芽）皮质的接触。在这里，我们建立了一系列新的事件，将Kar 9靶向芽皮层。首先，Kar 9在细胞周期的G（1）中与纺锤体极体（SPB）结合。其次，在G（1）/S中，酵母Cdk 1，Cdc 28与SPBs缔合并磷酸化Kar 9。第三，Kar 9和Cdc 28然后从SPB移动到CM的正端，朝向芽。这种运动依赖于微管马达蛋白Kip 2。Cdc 28的活性是集中Kar 9在CM的正端，从而建立与芽皮层的接触所必需的。因此，Cdc 28调节的Kar 9定位是对齐纺锤体的程序的组成部分。
The budding yeast spindle aligns along the mother-bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1, mediates contacts of CMs with the cortex of the daughter cell, the bud. Here we established a novel series of events that target Kar9 to the bud cortex. First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9. Thirdly, Kar9 and Cdc28 then move from the SPB to the plus end of CMs directed towards the bud. This movement is dependent upon the microtubule motor protein Kip2. Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles.