Behavior and the limits of genomic plasticity: power and replicability in microarray analysis of honeybee brains
Behavior and the limits of genomic plasticity: power and replicability in microarray analysis of honeybee brains
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DOI：
10.1111/j.1601-183x.2005.00131.x
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Robinson, GE
中科院分区：
文献类型：
--
作者：
Cash, AC;Whitfield, CW;Robinson, GE
相对于大脑中的许多转录后过程，转录是缓慢的。利用蜜蜂（Apis mellifera）丰富的劳动分工系统，我们发现不同持续时间的行为职业与大脑中基因表达差异相关的程度存在极大差异。护理和觅食，持续时间> 1周的职业，与近四分之一的测试基因（5563个cDNA中的1208个; P < 0.01，ANOVA）的显著表达差异相关，与先前的结果一致。相比之下，在哺乳后和觅食开始前1- 2天进行的过渡性职业，（守卫对生物标记; 19个cDNA，少于56个假阳性的预期）或很少的差异（梳状构建者对守卫和生物标记; 248个cDNA），但相对于哺乳和觅食两者存在广泛的差异（> 500个cDNA，所有对比）。统计功效分析表明，应分别在100%、92%和37%的病例中检测到2倍、1.5倍和1.25倍的表达差异。在这些量级上，先前结果的重复率为95%、71%和51%，没有基因显示出相反方向的差异。这些结果表明，在不同的时间尺度上的行为可塑性可能与大脑中基因组可塑性程度的实质性差异有关。
Transcription is slow relative to many post-transcriptional processes in the brain. Using the rich system of division of labor in the honeybee (Apis mellifera), we found extreme differences in the extent to which behavioral occupations of different durations were associated with gene-expression differences in the brain. Nursing and foraging, occupations lasting > 1 week, were associated with significant expression differences for nearly one-quarter of the genes tested (1208 of 5563 cDNAs tested; P < 0.01, ANOVA), consistent with previous results. In contrast, transitional occupations, performed for 1-2days after nursing and before the onset of foraging, were associated with either no differences (guards vs. undertakers; 19 cDNAs, fewer than the expectation of 56 false-positives) or few differences (comb builders vs. guards and undertakers; 248 cDNAs), but extensive differences relative to both nursing and foraging (> 500 cDNAs, all contrasts). Statistical power analysis indicated that expression differences of two-, 1.5- and 1.25-fold should have been detected in 100, 92 and 37% of cases, respectively. Replication of previous results at these magnitudes was 95, 71 and 51%, with no genes showing differences in the opposite direction. These results indicate that behavioral plasticity over different time-scales may be associated with substantial differences in the extent of genomic plasticity in the brain.