Two-photon imaging of microbial immunity in living tissues.
Two-photon imaging of microbial immunity in living tissues.
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DOI：
10.1017/s1431927612000281
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发表时间：
2012-08
影响因子：
2.8
通讯作者：
McGavern, Dorian B.
中科院分区：
文献类型：
--
作者：
Herz, Jasmin;Zinselmeyer, Bernd H.;McGavern, Dorian B.
免疫系统是高度进化的，可以对全身的感染作出反应。病原体特异性免疫细胞通常在次级淋巴组织（如脾脏、淋巴结）中产生，然后迁移到感染部位，在那里它们的功能受当地环境的影响。由于免疫细胞受其所在的感染组织的严重影响，因此在体内研究它们的相互作用和动力学是很重要的。双光子显微镜是研究活体组织中宿主-免疫相互作用的一种强有力的方法，最近该领域的技术进步使研究人员能够实时捕获免疫细胞和感染因子的电影。这些研究揭示了病原体通过完整组织的进入和传播，以及先天和适应性免疫细胞参与阻止感染的机制。本文综述了双光子显微镜如何用于研究体内组织特异性免疫反应，以及这种方法如何提高我们对感染后宿主-免疫相互作用的理解。
The immune system is highly evolved and can respond to infection throughout the body. Pathogen-specific immune cells are usually generated in secondary lymphoid tissues (e.g., spleen, lymph nodes) and then migrate to sites of infection where their functionality is shaped by the local milieu. Because immune cells are so heavily influenced by the infected tissue in which they reside, it is important that their interactions and dynamics be studied in vivo. Two-photon microscopy is a powerful approach to study host-immune interactions in living tissues, and recent technical advances in the field have enabled researchers to capture movies of immune cells and infectious agents operating in real time. These studies have shed light on pathogen entry and spread through intact tissues as well as the mechanisms by which innate and adaptive immune cells participate in thwarting infections. This review focuses on how two-photon microscopy can be used to study tissue-specific immune responses in vivo, and how this approach has advanced our understanding of host-immune interactions following infection.