Photoinduced force microscopy as a novel method for the study of microbial nanostructures.
Photoinduced force microscopy as a novel method for the study of microbial nanostructures.
复制标题
光诱导力显微镜作为研究微生物纳米结构的新方法。
DOI：
10.1039/d3nr03499b
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发表时间：
2023
期刊：
影响因子：
6.7
通讯作者：
Davies-Jones J
中科院分区：
文献类型：
--
作者：
Davies-Jones J
首次对电子显微镜和纳米红外（IR）显微镜成像微生物纳米结构的能力进行了详细的比较。表面的灵敏度，化学特异性，和非破坏性的性质，光谱映射显示提供显着的优势，透射电子显微镜（TEM）的生物样品的研究。以及产生重要的地形信息，酰胺，脂质和碳水化合物的分布在细菌（大肠杆菌，金黄色葡萄球菌）和真菌（白色念珠菌）细胞的横截面证明使用PiFM。从微生物细胞壁和膜的表面化学和生物学的光谱映射的这种新模式中获得的独特信息，可以提供对真菌/细菌细胞功能的新见解，以及在确定抗菌剂耐药性机制中具有潜在用途，特别是那些靶向细胞壁的机制。
A detailed comparison of the capabilities of electron microscopy and nano-infrared (IR) microscopy for imaging microbial nanostructures has been carried out for the first time. The surface sensitivity, chemical specificity, and non-destructive nature of spectroscopic mapping is shown to offer significant advantages over transmission electron microscopy (TEM) for the study of biological samples. As well as yielding important topographical information, the distribution of amides, lipids, and carbohydrates across cross-sections of bacterial (Escherichia coli, Staphylococcus aureus) and fungal (Candida albicans) cells was demonstrated using PiFM. The unique information derived from this new mode of spectroscopic mapping of the surface chemistry and biology of microbial cell walls and membranes, may provide new insights into fungal/bacterial cell function as well as having potential use in determining mechanisms of antimicrobial resistance, especially those targeting the cell wall.