Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes
Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes
复制标题
DOI：
10.1007/s00438-006-0167-z
复制
发表时间：
2006
影响因子：
3.1
通讯作者：
L. Larrondo;Paulo Canessa;R. Vicuña;P. Stewart;A. Vanden Wymelenberg;D. Cullen
中科院分区：
文献类型：
--
作者：
L. Larrondo;Paulo Canessa;R. Vicuña;P. Stewart;A. Vanden Wymelenberg;D. Cullen
我们描述了木质素降解担子菌黄孢原毛平革菌中重复元件的结构、组织和转录影响。对Thep的搜查。黄孢子菌基因组揭示了pce1的5个拷贝，pce1是一个~1,750个核苷酸的非自治的II类元件。编码一个假定的葡萄糖基转移酶和一个细胞色素P450的等位基因阻止插入并产生不完整的转录本。I类元件包括dpcret1，一个完整的8.14kbgypsy样反转录转座子，插入多铜氧化酶基因家族的一个成员中。此外，我们描述了嵌套转座子在另一个假定的细胞色素P450基因中的复杂插入。被破坏的等位基因位于一组>；14基因中，所有这些基因都编码家族的细胞色素P450。插入的成分包括分离拷贝样元件pcret3、第二反转录元件pcret2的极化结构域和未知功能的扩展ORF的复制。与pce元件的情况一样，pcret1和pcret2/3插入仅限于单个等位基因，其转录本被截断。相应的野生型等位基因显然不受影响。总体而言，黄孢原丝菌含有一系列复杂的重复元件，其中至少有五个元件直接影响结构相关序列家族中基因的表达。
We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete,Phanerochaete chrysosporium. Searches of theP. chrysosporiumgenome revealed five copies ofpce1, a ~1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harborpceinsertions and produce incomplete transcripts. Class I elements includedpcret1, an intact 8.14-kbgypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of >14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjointcopia-like element,pcret3, thepoldomain of a second retroelement,pcret2, and a duplication of an extended ORF of unknown function. As in the case of thepceelements,pcret1andpcret2/3insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaffected. In aggregate,P. chrysosporiumharbors a complex array of repetitive elements, at least five of which directly influence expression of genes within families of structurally related sequences.