Common principles in the biosynthesis of diverse enzymes
Common principles in the biosynthesis of diverse enzymes
复制标题
DOI：
10.1042/bst0330105
复制
发表时间：
2005-02-01
影响因子：
3.9
通讯作者：
Sargent, F
中科院分区：
文献类型：
--
作者：
Jack, RL;Dubini, A;Sargent, F
细菌周质酶的一个子集从细胞质中运输的双精氨酸运输装置。这些蛋白质含有独特的N-末端信号肽，其含有保守的SRRXFLK“双精氨酸”氨基酸基序，并且通常在转运事件之前结合复杂的辅因子。重要的是，含有复杂辅因子且通常是多亚基的酶的组装在输出之前完成。无关的[NiFe]氢化酶，DMSO还原酶和三甲胺N-氧化还原酶系统从大肠杆菌的研究使我们能够定义一个伴侣介导的“校对”机制参与协调装配和出口的双精氨酸转运依赖酶。
A subset of bacterial periplasmic enzymes are transported from the cytoplasm by the twin-arginine transport apparatus. Such proteins contain distinctive N-terminal signal peptides containing a conserved SRRXFLK 'twin-arginine' amino acid motif and often bind complex cofactors before the transport event. It is important that assembly of complex cofactor-containing, and often multi-subunit, enzymes is complete before export. Studies of the unrelated [NiFe] hydrogenase, DMSO reductase and trimethylamine N-oxide reductase systems from Escherichia coli have enabled us to define a chaperone-mediated 'proofreading' mechanism involved in co-ordinating assembly and export of twin-arginine transport-dependent enzymes.