Sub-diffusive scattering parameter maps recovered using wide-field high-frequency structured light imaging
Sub-diffusive scattering parameter maps recovered using wide-field high-frequency structured light imaging
复制标题
DOI：
10.1364/boe.5.003376
复制
发表时间：
2014-10-01
影响因子：
3.4
通讯作者：
Pogue, Brian W.
中科院分区：
文献类型：
--
作者：
Kanick, Stephen Chad;McClatchy, David M., III;Pogue, Brian W.
本文探讨了利用具有高空间频率图案(f(X))的结构光反射成像可以定量绘制混浊介质中各向异性散射相函数分布(P(theta(S)的假设。蒙特卡罗模拟部分用于建立解调反射率(R-d)的半经验模型，该模型以无因次散射(u(S)‘f(X)(-1))和伽马(P(theta(S))分布的前两个矩的度量)表示。在组织模拟体模中完成的实验表明，同时分析在频率范围[0.050.5]mm(-1)内以多个f(X)采样的R-d谱可以准确地估计相关组织范围[0.41.8]mm(-1)内的u(S)‘(Lambda)和范围[1.41.75]内的伽马(Lambda)。对一名健康志愿者的初步测量显示，疤痕组织和周围正常皮肤之间的伽马对比度在广域漫射成像中不太明显。这些结果代表了第一个用于量化亚弥散散射参数的广域图，这些参数对亚微观组织结构和组成很敏感，因此，为在与外科应用相关的大小尺度上快速诊断超微结构提供了可能性。(C)2014年美国光学学会
This study investigates the hypothesis that structured light reflectance imaging with high spatial frequency patterns (f(x)) can be used to quantitatively map the anisotropic scattering phase function distribution (P(theta(s))) in turbid media. Monte Carlo simulations were used in part to establish a semi-empirical model of demodulated reflectance (R-d) in terms of dimensionless scattering (mu(s)'f(x)(-1)) and gamma, a metric of the first two moments of the P(theta(s)) distribution. Experiments completed in tissue-simulating phantoms showed that simultaneous analysis of R-d spectra sampled at multiple f(x) in the frequency range [0.05-0.5] mm(-1) allowed accurate estimation of both mu(s)'(lambda) in the relevant tissue range [0.4-1.8] mm(-1), and gamma(lambda) in the range [1.4-1.75]. Pilot measurements of a healthy volunteer exhibited gamma-based contrast between scar tissue and surrounding normal skin, which was not as apparent in wide field diffuse imaging. These results represent the first wide-field maps to quantify sub-diffuse scattering parameters, which are sensitive to sub-microscopic tissue structures and composition, and therefore, offer potential for fast diagnostic imaging of ultrastructure on a size scale that is relevant to surgical applications. (C) 2014 Optical Society of America