Transformed glucocorticoid receptors consist of multiple subspecies with differing capacities to bind DNA-cellulose.
Transformed glucocorticoid receptors consist of multiple subspecies with differing capacities to bind DNA-cellulose.
复制标题
转化的糖皮质激素受体由具有不同 DNA-纤维素结合能力的多个亚种组成。
DOI：
10.1021/bi00433a028
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Wolfe,KA
中科院分区：
文献类型：
--
作者：
Gruol,DJ;Wolfe,KA
Donald J. Gruol** and Kristal A. Wolfe The Salk Institute for Biological Studies, San Diego, California 92138 Received September 1, 1988; Revised Manuscript Received December 1, 1988 abstract: The glucocorticoid receptor can be transformed into a DNA-binding protein by a process that is both hormone and temperature dependent. We have used a modification of the conventional method of anion-exchange chromatography to separate and analyze a variety of receptor subspecies that result from this transition. One receptor form (peak A) was found to have a capacity to bind DNA-cellulose which was significantly greater than that of the other species. Under conditions of mild heating (15 C), the relative abundance of peak A in the receptor population and the rate of receptor transformation were both increased as a result of incubating samples with alkaline phosphatase. The mechanism appears to involve the conversion of the more “acidic” forms into that of peak A. The results indicate that receptor transformation is a multistep process which may be promoted by the removal of phosphate from either the receptor or a receptor-bound regulatory factor.
