Photoregulation of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) in the accumulation of anthocyanin in alpine and prairie ecotypes of Stellaria longipes under varied R/FR
Photoregulation of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) in the accumulation of anthocyanin in alpine and prairie ecotypes of Stellaria longipes under varied R/FR
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DOI：
10.1034/j.1399-3054.2002.1160412.x
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发表时间：
2002-12-01
影响因子：
6.4
通讯作者：
Reid, DM
中科院分区：
文献类型：
--
作者：
Alokam, S;Li, YJ;Reid, DM
研究了高寒和草原生态型龙参苯丙氨酸解氨酶（PAL）和查尔酮合成酶（CHS） mRNA的积累和酶活性。结果表明，光对诱导PAL和CHS以及花青素的积累是必需的。尽管两种生态型在高R/FR（1.9）条件下花青素水平均高于低R/FR（0.7）条件下的花青素水平，但两种生态型在低R/FR和高R/FR条件下PAL或CHS转录物丰度均无显著差异。然而，两种生态型在高R/FR条件下的CHS活性均显著高于低R/FR条件下的CHS活性。这些结果表明，在不同的R/FR条件下，长叶参CHS活性对花青素合成的调控比PAL更具特异性，且酶活性与花青素积累之间存在一定的时滞。
We studied mRNA accumulation and enzyme activity of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) in alpine and prairie ecotypes of Stellaria longipes. The results show that light is required for the induction of PAL and CHS and accumulation of anthocyanins. Although both the ecotypes showed higher levels of anthocyanins under high R/FR (1.9) than under low R/FR (0.7), neither of the ecotypes showed any significant difference in the transcript abundance of PAL or CHS between low R/FR and high R/FR. However, both ecotypes showed significantly higher levels of CHS activity under high R/FR than under low R/FR corresponding to their anthocyanin accumulation. These results indicate that in S. longipes regulation of CHS activity is more specific to anthocyanin synthesis than PAL under varied R/FR, with a time lag between the enzyme activity and the accumulation of anthocyanins.