Simultaneous multiresidue determination of tetracyclines and fluoroquinolones in catfish muscle using high performance liquid chromatography with fluorescence detection
Simultaneous multiresidue determination of tetracyclines and fluoroquinolones in catfish muscle using high performance liquid chromatography with fluorescence detection
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DOI：
10.1016/j.aca.2006.09.025
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发表时间：
2007-03-14
影响因子：
6.2
通讯作者：
Freeman, Donald W.
中科院分区：
文献类型：
--
作者：
Schneider, Marilyn J.;Darwish, Ahmed M.;Freeman, Donald W.
食品中兽药残留的分析需要高效的方法。许多方法可用于单一分析物。多残留方法现在越来越多地可用。然而，仍然很少发现不涉及质谱法的方法，允许分析一种以上的药物残留。建立了一种同时测定鲶鱼肌肉中氟喹诺酮类和四环素类药物残留的高效多残留分析方法。该方法涉及用乙腈和含有氯化镁的柠檬酸盐缓冲液的混合物提取分析物。离心和蒸发上清液后，使用高效液相色谱法和荧光检测法测定残留物。用这种方法，五氟喹诺酮类和四环素类抗生素在强化鲶鱼肌肉中测定水平为20，50，和100 ng g（-1）。环丙沙星（CIP）、沙拉沙星（SAR）、达氟沙星（DANO）、恩诺沙星（ENRO）、二氟沙星（DIF）、土霉素（OTC）、四环素（TC）和金霉素（CTC）的平均回收率在60-92%范围内，相对标准偏差良好。定量限范围为0.15至1.5 ng g（-1）。利用该方法成功地分析了鲶鱼肌肉样品与恩诺沙星和土霉素。(c)2006 Elsevier B. V.保留所有权利。
Efficient methods are needed for analysis of veterinary drug residues in food. A number of methods are available for single analytes. Multiresidue methods are now increasingly available. It is still rare, however, to find methods not involving mass spectrometry which allow for analysis of more than one class of drug residue. An efficient multiresidue method for the simultaneous determination of fluoroquinolones (FQs) and tetracyclines (TCs) in catfish muscle has now been developed. This method involves an extraction of the analytes with a mixture of acetonitrile and citrate buffer containing magnesium chloride. After centrifugation and evaporation of the supernatants, the residues are determined using high performance liquid chromatography with fluorescence detection. With this method, five fluoroquinolones and three tetracyclines were determined in fortified catfish muscle at levels of 20, 50, and 100 ng g(-1). Average recoveries for ciprofloxacin (CIP), sarafloxacin (SAR), danofloxacin (DANO), enrofloxacin (ENRO), difloxacin (DIF), oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were in the range of 60-92% with good relative standard deviations. The limits of quantitation ranged from 0.15 to 1.5 ng g(-1). Utilization of the method to successfully analyze catfish muscle samples incurred with enrofloxacin and with oxytetracycline is described. (c) 2006 Elsevier B.V. All rights reserved.