Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Quantitative IR microscopy and spectromics open the way to 3D digital pathology
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DOI：
10.1002/jbio.201600051
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发表时间：
2017-04-01
影响因子：
2.8
通讯作者：
Petibois, Cyril
中科院分区：
文献类型：
--
作者：
Bobroff, Vladimir;Chen, Hsiang-Hsin;Petibois, Cyril
目前，只有质谱（MS）显微镜可以对3D组织样本的化学成分进行定量分析。在这里，一个三维定量化学图像的生物组织的FTIR光谱显微镜重建报告。提出了一种自动曲线拟合的方法来提取构成红外光谱的所有强吸收带。这项创新得益于三个关键特征：（1）校正原始IR光谱，使其具有定量可比性;（2）自动化和迭代数据处理，允许将IR吸收光谱转换为IR波段光谱;（3）重建3D IR波段矩阵（x，y，z表示体素位置，4维表示所有IR波段参数）。Spectrometry是一种利用光谱数据进行组织元数据重建的新方法，它可以进一步将相关的化学信息转化为3D的生化和解剖组织参数。一个例子是与氧化应激分布和组织中的血管重建。红外显微镜仪器的要求，提出3D数字组织学作为临床常规技术进行了简要讨论。
Currently, only mass-spectrometry (MS) microscopy brings a quantitative analysis of chemical contents of tissue samples in 3D. Here, the reconstruction of a 3D quantitative chemical images of a biological tissue by FTIR spectro-microscopy is reported. An automated curve-fitting method is developed to extract all intense absorption bands constituting IR spectra. This innovation benefits from three critical features: (1) the correction of raw IR spectra to make them quantitatively comparable; (2) the automated and iterative data treatment allowing to transfer the IR-absorption spectrum into a IR-band spectrum; (3) the reconstruction of an 3D IR-band matrix (x, y, z for voxel position and a 4(th) dimension with all IR-band parameters). Spectromics, which is a new method for exploiting spectral data for tissue metadata reconstruction, is proposed to further translate the related chemical information in 3D, as biochemical and anatomical tissue parameters. An example is given with oxidative stress distribution and the reconstruction of blood vessels in tissues. The requirements of IR microscopy instrumentation to propose 3D digital histology as a clinical routine technology is briefly discussed.