Aggregation and loss of cytokeratin filament networks inhibit golgi organization in liver-derived epithelial cell lines
Aggregation and loss of cytokeratin filament networks inhibit golgi organization in liver-derived epithelial cell lines
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DOI：
10.1002/cm.10152
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发表时间：
2004-01-01
影响因子：
--
通讯作者：
Sata, M
中科院分区：
文献类型：
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作者：
Kumemura, H;Harada, M;Sata, M
中间丝是三种主要的细胞骨架之一。基于与细胞角蛋白突变相关的各种疾病，已经出现了中间丝在细胞功能中的一些作用。然而，中间丝的确切功能仍然不清楚。为了解决这个问题，我们操纵中间丝的培养细胞表达突变体细胞角蛋白。细胞角蛋白18的精氨酸89在中间丝组装中起重要作用。绿色荧光蛋白标记的细胞角蛋白18 arg 89 cys的表达诱导肝源性上皮细胞Huh 7和OUMS 29中由细胞角蛋白组成的中间丝网络的聚集和损失，但仅诱导细胞角蛋白聚集体的形成，并且不影响HEK 293中内源性波形蛋白的中间丝网络。在Huh 7和OUMS 29中，该突变体的表达影响高尔基体的分布和高尔基体在诺考达唑或布雷菲德菌素A扰动后的重新组装，但在HEK 293中不影响。我们的数据表明，损失的原始中间丝网络，但不存在的细胞角蛋白聚集体，诱导重新分配的高尔基体。原始完整的中间丝网络是高尔基体组织所必需的。(C)2003 Wiley-Liss，Inc.
Intermediate filaments are one of the three major cytoskeletons. Some roles of intermediate filaments in cellular functions have emerged based on various diseases associated with mutations of cytokeratins. However, the precise functions of intermediate filament are still unclear. To resolve this, we manipulated intermediate filaments of cultured cells by expressing a mutant cytokeratin. Arginine 89 of cytokeratin18 plays an important role in intermediate filament assembly. The expression of green fluorescent protein-tagged cytokeratin 18 arg89cys induced aggregations and loss of the intermediate filament network composed of cytokeratins in liver-derived epithelial cells, Huh7 and OUMS29, but only induced the formation of cytokeratin aggregates and did not affect the intermediate filament network of endogenous vimentin in HEK293. The expression of this mutant affected the distribution of Golgi apparatus and the reassembly of Golgi apparatus after perturbations by nocodazole or brefeldin A in both Huh7 and OUMS29, but not in HEK293. Our data show that loss of the original intermediate filament network, but not the existence of cytokeratin aggregates, induces redistribution of the Golgi apparatus. The original intact intermediate filament network is necessary for the organization of Golgi apparatus. (C) 2003 Wiley-Liss, Inc.