The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum
The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum
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DOI：
10.1111/j.1365-313x.2008.03651.x
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发表时间：
2008-12-01
期刊：
影响因子：
7.2
通讯作者：
Somssich, Imre E.
中科院分区：
文献类型：
--
作者：
Mukhtar, M. Shahid;Deslandes, Laurent;Somssich, Imre E.
WRKY转录因子在调节植物防御转录组中起关键作用。在这里，我们表明，拟南芥突变体wrky 27 -1，它缺乏一个功能性WRKY 27转录因子，表现出延迟症状的发展，响应青枯病病原菌青枯菌。另外，wrky 27 -1植物在感染后不表达PR标记基因，如在抗性Nd-1植物中也观察到的。WRKY 27的空间表达与细菌的感染途径和在植物中的繁殖密切相关。互补实验恢复了野生型Col-1植物的早期萎蔫表型和PR基因的激活，不仅当WRKY 27 cDNA在天然启动子的控制下表达时，而且当使用SUC 2启动子时，表明WRKY 27在韧皮部伴随细胞中发挥其功能。表达研究确定了参与氮代谢和一氧化氮（NO）生成的基因作为WRKY 27负调控的潜在靶点。我们的研究结果表明，WRKY 27负面影响症状发展的维管病原体，可能是通过影响信号或韧皮部和木质部之间的运输。
WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Additionally, wrky27-1 plants did not express PR marker genes upon infection, as also observed in resistant Nd-1 plants. Spatial expression of WRKY27 correlated well with the route of bacterial infection and propagation in planta. Complementation experiments restored both the early wilting phenotype of wild-type Col-1 plants and activation of PR genes, not only when the WRKY27 cDNA is expressed under the control of the native promoter, but also when the SUC2 promoter was used, suggesting that WRKY27 exerts its function in phloem companion cells. Expression studies identified genes involved in nitrogen metabolism and nitric oxide (NO) generation as potential targets of negative regulation by WRKY27. Our results show that WRKY27 negatively influences symptom development of a vascular pathogen, possibly by affecting signaling or trafficking between the phloem and the xylem.