Stress distributions and cell flows in a growing cell aggregate
Stress distributions and cell flows in a growing cell aggregate
复制标题
DOI：
10.1098/rsfs.2014.0033
复制
发表时间：
2014-12-06
期刊：
影响因子：
4.4
通讯作者：
Prost, Jacques
中科院分区：
文献类型：
--
作者：
Delarue, Morgan;Joanny, Jean-Francois;Prost, Jacques
我们讨论了多细胞球体对外部压力跳变的短时响应。我们的实验表明，在压力跳跃后5分钟，球体中心的细胞密度增加，但靠近球体表面的细胞密度没有明显变化。这个结果可以解释，如果细胞是极化的，我们显示的情况。受实验结果的启发，我们发展了极化球体的理论，其中电池极性是径向的（除了在靠近球体表面的薄壳中）。该理论考虑了细胞分裂率和凋亡率对局部应激的依赖性，细胞产生的细胞极性和主动应激以及主动应激对局部应激的依赖性。我们发现，在压力跳变后，电池密度在短时间内增加，并以幂律形式从球体中心衰减，这与实验结果基本一致。通过与实验的比较，我们可以估计出组织的各向同性压缩模量。
We discuss the short-time response of a multicellular spheroid to an external pressure jump. Our experiments show that 5 min after the pressure jump, the cell density increases in the centre of the spheroid but does not change appreciably close to the surface of the spheroid. This result can be explained if the cells are polarized which we show to be the case. Motivated by the experimental results, we develop a theory for polarized spheroids where the cell polarity is radial (except in a thin shell close to the spheroid surface). The theory takes into account the dependence of cell division and apoptosis rates on the local stress, the cell polarity and active stress generated by the cells and the dependence of active stress on the local pressure. We find a short-time increase of the cell density after a pressure jump that decays as a power law from the spheroid centre, which is in reasonable agreement with the experimental results. By comparing our theory to experiments, we can estimate the isotropic compression modulus of the tissue.