Spliceosome-associated factor CTNNBL1 promotes proliferation and invasion in ovarian cancer
Spliceosome-associated factor CTNNBL1 promotes proliferation and invasion in ovarian cancer
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剪接体相关因子 CTNNBL1 促进卵巢癌的增殖和侵袭
DOI：
10.1016/j.yexcr.2017.05.008
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发表时间：
2017-08-01
影响因子：
3.7
通讯作者：
Kong, Beihua
中科院分区：
文献类型：
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作者：
Li, Yingwei;Guo, Haiyang;Kong, Beihua
卵巢癌是最致命的妇科恶性肿瘤，高级别浆液性卵巢癌的分子发病机制尚未完全确定。许多研究表明，改变的剪接模式和剪接因子被发现有助于肿瘤的发展和进展。在本研究中，我们证明剪接体相关因子CTNNBL 1在高级别浆液性卵巢癌中显著上调，CTNNBL 1水平升高表明高级别浆液性卵巢癌患者预后不良。CTNNBL 1在体外可促进卵巢癌细胞的增殖和侵袭。此外，通过转录组分析，我们发现CTNNBL 1调节卵巢癌细胞中的多个剪接事件和基因表达。重要的是，我们确定IF116和FOXM1剪接受CTNNBL 1调控。据我们所知，这是第一个研究探讨CTNNBL 1在卵巢癌中的表达，功能作用和调节剪接事件。
Ovarian cancer is the most lethal gynecologic malignancy and the molecular pathogenesis of high-grade serous ovarian carcinoma has not been completely characterized. Numerous studies have shown that altered splicing patterns and splicing factors were found to contribute to tumor development and progression. In this study, we demonstrated that spliceosome-associated factor CTNNBL1 was significantly upregulated in high-grade serous ovarian carcinoma, the elevated level of CTNNBL1 indicates poor prognosis in patients with high-grade serous ovarian carcinoma. Functional characterization revealed that CTNNBL1 promoted the proliferation and invasion of ovarian cancer cells in vitro. Furthermore, through transcriptome analysis, we found CTNNBL1 regulates multiple splicing events and gene expression in ovarian cancer cells. Importantly, we identified IF116 and FOXM1 splicing was regulated by CTNNBL1. To our knowledge, this is the first study exploring the expression, functional roles and regulated splicing events of CTNNBL1 in ovarian cancer.