Establishment and characterization of the growth and pulmonary metastasis of a highly lung metastasizing cell line from canine osteosarcoma in nude mice.
Establishment and characterization of the growth and pulmonary metastasis of a highly lung metastasizing cell line from canine osteosarcoma in nude mice.
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裸鼠犬骨肉瘤高度肺转移细胞系的生长和肺转移的建立和表征。
DOI：
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发表时间：
1999
影响因子：
1.2
通讯作者：
T. Fujinaga
中科院分区：
文献类型：
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作者：
Edward F. Barroga;T. Kadosawa;Masahiro Okumura;T. Fujinaga
通过5个肺肿瘤沉积周期皮下移植，建立了裸鼠犬骨肉瘤高肺转移模型。从亲本犬骨肉瘤细胞系中选择转移性增加的细胞，恢复了中等大小和多边形高度转移性骨肉瘤细胞（HMPOS）。HMPOS和POS在体外培养的平均倍增时间分别为30 +/- 1.2 hr和32 +/- 1.3 hr，其细胞在体外的生长模式与体内的生长模式相当。植入后12周，HMPOS细胞在置换肺组织后产生了更多不同大小的肿瘤沉积物（bbb20个结节，>直径为1 -mm）。POS细胞产生较少和较小的肺沉积物（< 10个结节，直径1毫米）。肿瘤的大小与转移性肿瘤的数量有一定的相关性。HMPOS细胞在皮下和肺中向成骨细胞型分化。HMPOS微转移沿肺泡壁和血管4周平均6-7个小肿瘤位点。每个微转移位点平均包含5-7个肿瘤细胞，并发展为多形性成骨细胞型细胞分化。6周时平均可见4个大转移结节，其中平均23个肿瘤细胞，8周时10个，10周时12个，12周时20个。该模型为评价裸鼠肺转移性骨肉瘤的新治疗方法提供了机会。
Highly lung metastasizing model of canine osteosarcoma in nude mice was established from five subcutaneous implantation cycles of lung tumor deposits. The selection of cells with increased metastatic properties from the parent POS canine osteosarcoma cell line recovered medium sized and polygonal Highly Metastasizing POS cells (HMPOS). The doubling time of HMPOS and POS in culture averaged 30 +/- 1.2 hr and 32 +/- 1.3 hr respectively, and their cell growth patterns in vitro were comparable to their in vivo growth patterns. HMPOS cells produced more tumor deposits (> 20 nodules, > 1 -mm in diameter) of various sizes with replacement of lung tissues at 12 weeks after implantation. POS cells produced fewer and smaller lung deposits (< 10 nodules, 1-mm in diameter). Tumor size and number of metastatic tumor deposits showed a regular association. HMPOS cells developed an osteoblastic type of cellular differentiation subcutaneously and in the lungs. HMPOS micrometastasis along the alveolar walls and blood vessels at 4 weeks averaged 6-7 small tumor locus. Each micrometastatic locus contained an average of 5-7 tumor cells, and developed a pleomorphic osteoblastic type of cellular differentiation. An average of 4 macrometastatic nodules could be seen at 6 weeks, composed of an average of 23 tumor cells, 10 nodules at 8 weeks, 12 nodules at 10 weeks and 20 nodules at 12 weeks. These model provides an opportunity for the evaluation of new treatments against canine lung metastatic osteosarcoma in a nude mice model.
影响因子：
11.2
作者：
K. Morikawa;S. Walker;J. Jessup;I. Fidler
通讯作者：
K. Morikawa;S. Walker;J. Jessup;I. Fidler