Recent advancement of light-based single-molecule approaches for studying biomolecules
Recent advancement of light-based single-molecule approaches for studying biomolecules
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DOI：
10.1002/wsbm.1445
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发表时间：
2019-07-01
影响因子：
7.9
通讯作者：
Han, Kyu Young
中科院分区：
文献类型：
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作者：
Croop, Benjamin;Zhang, Chenyi;Han, Kyu Young
单分子技术的最新进展导致了分析化学，生物物理学和医学的新发现。与研究大型集合体相比，了解单个生物分子的结构和行为提供了丰富的信息。然而，开发单分子技术是具有挑战性的，需要在光学，工程，生物学和化学方面取得进展。在本文中，我们将回顾单分子应用的最新技术水平，重点是过去几年的发展。所涵盖的进步将主要包括基于光的体外方法，我们将讨论每种方法的基本原理，重点是平台本身。我们还将总结它们的局限性以及当前和未来在更广泛的生物和化学领域的应用。本文分类下：实验室方法和技术>成像实验室方法和技术>高分子相互作用，方法分析和计算方法>分析方法
Recent advances in single-molecule techniques have led to new discoveries in analytical chemistry, biophysics, and medicine. Understanding the structure and behavior of single biomolecules provides a wealth of information compared to studying large ensembles. However, developing single-molecule techniques is challenging and requires advances in optics, engineering, biology, and chemistry. In this paper, we will review the state of the art in single-molecule applications with a focus over the last few years of development. The advancements covered will mainly include light-based in vitro methods, and we will discuss the fundamentals of each with a focus on the platforms themselves. We will also summarize their limitations and current and future applications to the wider biological and chemical fields. This article is categorized under: Laboratory Methods and Technologies > Imaging Laboratory Methods and Technologies > Macromolecular Interactions, Methods Analytical and Computational Methods > Analytical Methods