Super resolution NOESY spectra of proteins
Super resolution NOESY spectra of proteins
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DOI：
10.1007/s10858-019-00231-x
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发表时间：
2019-04-01
影响因子：
2.7
通讯作者：
Lorieau, Justin L.
中科院分区：
文献类型：
--
作者：
DeLisle, Charles F.;Mendis, H. Bhagya;Lorieau, Justin L.
在生物分子的NMR研究中，光谱分辨率仍然是最重要的限制之一。介绍了超分辨核奥弗豪泽效应谱（srNOESY）实验，该实验以牺牲对角峰线宽为代价，提高了NOESY交叉峰的分辨能力。我们研究了两种蛋白质，泛素和流感血凝素融合肽在bicelles，我们实现了21-47%的平均分辨率增强和个别峰值增强高达约。百分之四百五十作为这些改进的结果，在两种蛋白质的常规NOESY实验中观察到新的峰，并且从计算的约束产生的最终结构与已发表的模型相匹配。我们讨论了实验参数，自旋扩散和srNOESY线型的信息含量的影响。
Spectral resolution remains one of the most significant limitations in the NMR study of biomolecules. We present the srNOESY (super resolution nuclear overhauser effect spectroscopy) experiment, which enhances the resolution of NOESY cross-peaks at the expense of the diagonal peak line-width. We studied two proteins, ubiquitin and the influenza hemagglutinin fusion peptide in bicelles, and we achieved average resolution enhancements of 21-47% and individual peak enhancements as large as ca. 450%. New peaks were observed over the conventional NOESY experiment in both proteins as a result of these improvements, and the final structures generated from the calculated restraints matched published models. We discuss the impact of the experimental parameters, spin diffusion and the information content of the srNOESY lineshape.