Design of a portable imager for near-infrared visualization of cutaneous wounds.
Design of a portable imager for near-infrared visualization of cutaneous wounds.
复制标题
设计用于皮肤伤口近红外可视化的便携式成像仪。
DOI：
10.1117/1.jbo.22.1.016010
复制
发表时间：
2017
影响因子：
3.5
通讯作者：
Hu,Wenjing
中科院分区：
文献类型：
--
作者：
Peng,Zhaoqiang;Zhou,Jun;Dacy,Ashley;Zhao,Deyin;Kearney,Vasant;Zhou,Weidong;Tang,Liping;Hu,Wenjing
描述了一种便携式成像仪，用于在研究环境中对皮肤伤口进行实时成像。该成像仪由一个高分辨率的近红外CCD摄像机组成，能够检测生物发光和荧光，由一个可旋转的滤光轮的LED环照明。所有外部组件都集成到一个紧凑的相机附件中。该设备被证明具有竞争力的性能与商业动物成像封闭箱设置在光束均匀性和灵敏度。具体而言，该器械用于在皮肤伤口炎症模型中观察伤口愈合过程中与活性氧活性增加相关的生物发光。此外，该装置被用来观察与基质金属蛋白酶在小鼠脂多糖诱导的感染模型中的活性相关的荧光。我们的研究结果支持使用便携式成像仪设计作为一种非侵入性和实时成像工具，以评估伤口炎症和感染的程度。
A portable imager developed for real-time imaging of cutaneous wounds in research settings is described. The imager consists of a high-resolution near-infrared CCD camera capable of detecting both bioluminescence and fluorescence illuminated by an LED ring with a rotatable filter wheel. All external components are integrated into a compact camera attachment. The device is demonstrated to have competitive performance with a commercial animal imaging enclosure box setup in beam uniformity and sensitivity. Specifically, the device was used to visualize the bioluminescence associated with increased reactive oxygen species activity during the wound healing process in a cutaneous wound inflammation model. In addition, this device was employed to observe the fluorescence associated with the activity of matrix metalloproteinases in a mouse lipopolysaccharide-induced infection model. Our results support the use of the portable imager design as a noninvasive and real-time imaging tool to assess the extent of wound inflammation and infection.