Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family
Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family
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DOI：
10.1006/geno.2000.6168
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发表时间：
2000-05-01
期刊：
影响因子：
4.4
通讯作者：
Peterson, P
中科院分区：
文献类型：
--
作者：
Aapola, U;Shibuya, K;Peterson, P
我们分离出了与胞嘧啶-5-甲基转移酶 3 (DNMT3) 家族相关的 DNMT3L 基因。该基因位于 AIRE 和 KIAA0653 基因之间的染色体 21q22.3 上，跨越约 16 kb 的基因组序列。编码的 387 个氨基酸的蛋白具有富含半胱氨酸的区域，其中包含新型锌指结构域，该结构域在 DNMT3A 和 DNMT3B 中保守，而且在 SNF2 蛋白家族成员 ATRX 中也保守。该新型结构域称为 ADD（ATRX、DNMT3、DNMT3L）型锌指，包含两个子部分：C2C2 和不完美的 PHD 锌指。 Northern blotting 无法检测到 DNMT3L mRNA 的表达；然而，RT-PCR 扩增表明它在睾丸、卵巢和胸腺等多种组织中低水平表达。 (C) 2000 年学术出版社。
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.