Engineering Peptide-based Carriers for Drug and Gene Delivery
Engineering Peptide-based Carriers for Drug and Gene Delivery
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DOI：
10.1007/978-1-4471-4372-7_25
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发表时间：
2014
影响因子：
8.6
通讯作者：
Jo-Ann Chuah;D. Kaplan;K. Numata
中科院分区：
文献类型：
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作者：
Jo-Ann Chuah;D. Kaplan;K. Numata
最近的研究主要集中在优化细胞递送系统，以提高细胞特异性，结合细胞器靶向和提高整体递送效率。多肽和蛋白质代表了满足这些目标的新的创新策略。多肽的优点是多方面的：它们可以将DNA凝聚成致密的颗粒进行运输，破坏内体膜，逃避蛋白酶体降解，将各种大小、电荷和功能的治疗分子运输到靶向细胞内室，并且可以降低细胞毒性和免疫原性。这些性质可以是单个肽的一部分，也可以是不同肽偶联的结果。丝绸是一种结构蛋白，以其生物可降解性和生物相容性而闻名，可以通过基因工程来定制特定的设计特征。由于源自蜘蛛和昆虫的丝蛋白具有可调节的结构、化学和机械性能，修饰或重组丝蛋白可用于各种生物医学应用，例如基因传递系统的设计。本文综述了多肽和丝蛋白在介导基因和药物细胞内传递中的多样性及其应用。
Recent research efforts have focused on the optimization of cell delivery systems with the aims of increasing cell specificity, incorporating organelle targeting and improving overall delivery efficiency. Peptides and proteins represent new and innovative strategies to meet these targets. The advantages of peptides are manyfold: they can condense DNA into compact particles for transport, disrupt the endosomal membrane, escape proteasomal degradation, traffic therapeutic molecules of various size, charge, and function to targeted intracellular compartments, and can have reduced cytotoxicity and immunogenicity. These properties can be part of a single peptide or the result from the conjugation of different peptides. Silk, a structural protein, is well known for its biodegradability and biocompatibility and can be tailored for specific design features via genetic engineering. With tunable structure, chemistry, and mechanical properties for silk proteins derived from spiders and insects, modified or recombinant silk proteins can be utilized in various biomedical applications such as for the design of gene delivery systems. This review summarizes the diversity and application of peptides and silk proteins to mediate intracellular delivery of genes and drugs.