Variation in gene expression within and among natural populations
Variation in gene expression within and among natural populations
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DOI：
10.1038/ng983
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发表时间：
2002-10-01
期刊：
影响因子：
30.8
通讯作者：
Crawford, DL
中科院分区：
文献类型：
--
作者：
Oleksiak, MF;Churchill, GA;Crawford, DL
进化可能更强烈地依赖于基因表达的变化，而不是蛋白质变体形式之间的差异（1）。影响基因表达的DNA区域高度可变，含有0.6%的多态性位点（2）。这些天然存在的多态性核苷酸可以改变体内转录速率（3 - 7）。因此，人们可以预期个体之间的基因表达存在显著差异。但是大量基因mRNA表达的自然变异还没有被测量。在这里，我们报告的微阵列研究解决基因表达的变化内和自然种群之间的硬骨鱼属底。我们观察到907个基因中约18%的基因在同一人群中个体之间的表达存在统计学显著差异。表达通常相差1.5倍，通常超过2.0倍。种群间的差异增加了变异。种群间的变异大部分是种群内变异的正函数，因此最简单地说是随机的。一些基因表现出意想不到的表达模式--与进化距离无关的变化。这些数据表明，大量的自然变异存在于基因表达，这种数量上的变化是重要的进化。
Evolution may depend more strongly on variation in gene expression than on differences between variant forms of proteins(1). Regions of DNA that affect gene expression are highly variable, containing 0.6% polymorphic sites(2). These naturally occurring polymorphic nucleotides can alter in vivo transcription rates(3-7). Thus, one might expect substantial variation in gene expression between individuals. But the natural variation in mRNA expression for a large number of genes has not been measured. Here we report microarray studies addressing the variation in gene expression within and between natural populations of teleost fish of the genus Fundulus. We observed statistically significant differences in expression between individuals within the same population for approximately 18% of 907 genes. Expression typically differed by a factor of 1.5, and often more than 2.0. Differences between populations increased the variation. Much of the variation between populations was a positive function of the variation within populations and thus is most parsimoniously described as random. Some genes showed unexpected patterns of expression-changes unrelated to evolutionary distance. These data suggest that substantial natural variation exists in gene expression and that this quantitative variation is important in evolution.