Increased collagen synthesis by scleroderma skin fibroblasts in vitro: a possible defect in the regulation or activation of the scleroderma fibroblast.
Increased collagen synthesis by scleroderma skin fibroblasts in vitro: a possible defect in the regulation or activation of the scleroderma fibroblast.
复制标题
体外硬皮病皮肤成纤维细胞胶原合成增加：硬皮病成纤维细胞的调节或激活可能存在缺陷。
DOI：
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发表时间：
1974
影响因子：
15.9
通讯作者：
E. Leroy
中科院分区：
文献类型：
--
作者：
E. Leroy
从正常皮肤和硬化皮肤中分离的皮肤成纤维细胞的培养可以估计可溶性胶原蛋白浓度、净胶原蛋白积累、细胞加倍次数以及形态和超微结构特征的比较。在体外，硬皮病成纤维细胞产生更多的可溶性胶原蛋白，更快地合成胶原蛋白，其蛋白质合成活性是正常皮肤成纤维细胞的四倍。细胞加倍次数和培养细胞的形态和超微结构观察并没有为硬皮病成纤维细胞调节或激活胶原合成的生物学缺陷的性质提供线索。
Cultures of dividing skin fibroblasts from normal and sclerodermatous human skin have permitted estimations of soluble collagen concentration, net collagen accumulation, cell-doubling times, and the comparison of morphologic and ultrastructural characteristics. In vitro, the scleroderma fibroblast produces more soluble collagen, synthesizes collagen more rapidly, and fourfold more of its protein synthetic activity is directed to collagen production than in the normal skin fibroblast. Cell-doubling times and morphologic and ultrastructural observations of cells in culture have not provided clues to the nature of the biologic defect in the regulation or activation of collagen synthesis by the scleroderma fibroblast.