5-HT is associated with the dysfunction of regulating T cells in patients with allergic rhinitis.
5-HT is associated with the dysfunction of regulating T cells in patients with allergic rhinitis.
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5-HT与过敏性鼻炎患者调节T细胞功能障碍有关。
DOI：
10.1016/j.clim.2022.109101
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发表时间：
2022-08
期刊：
影响因子：
--
通讯作者：
Pingchang Yang
中科院分区：
文献类型：
--
作者：
Gui Yang;Gaohui Wu;Wenkai Yao;Li Guan;Xiaorui Geng;Jiangqi Liu;Zhiqiang Liu;Liteng Yang;Qinmiao Huang;Xianhai Zeng;Pingchang Yang
调节性T淋巴细胞（Treg）功能异常与多种疾病的发生有关。5-羟色胺（5-HT）能够与免疫细胞相互作用。本研究的目的是阐明5-HT在调节Treg活性中的作用。从常年性变应性鼻炎（AR）患者中采集血样。通过磁性细胞分选从血液样品中分离出T细胞。采用酶联免疫吸附法测定5-HT和其他细胞因子的水平。结果表明，AR患者血清5-HT水平高于健康对照组（HC）。血清中5-HT浓度和AR相关细胞因子浓度之间的数据呈正相关。血清5-HT水平与外周血Treg频率呈负相关。5-HT可增强树突状细胞（DC）IL-6和IL-21的表达。5-HT-致敏的DC与T细胞共培养导致Th 17细胞的转化。STAT 3阻断有效地消除了5-HT相关的Th 17细胞从TCFs的转化。总之，AR患者血清5-HT浓度较高。5-HT致敏的DC可将Tcl 4转化为Th 17细胞。
The dysfunction of regulating T lymphocytes (Treg) is associated with the pathogenesis of many diseases. 5-hydroxytryptamine (5-HT) is capable of interacting with immune cells. The objective of the present study is to shed light on the role of 5-HT in regulating Treg activities. Blood samples were collected from patients with perennial allergic rhinitis (AR). Tregs were isolated from blood samples by magnetic cell sorting. The levels of 5-HT and other cytokines were determined by enzyme-linked immunosorbent assay. The results showed that serum 5-HT levels in patients with AR were higher than in healthy control (HC) subjects. A positive correlation was identified in the data between 5-HT concentrations and AR-related cytokine concentrations in the serum. A negative correlation was found between serum levels of 5-HT and the peripheral frequency of Treg. Exposure to 5-HT enhanced the expression of IL-6 and IL-21 in dendritic cells (DC). Co-culture of 5-HT-primed DCs with Tregs led to the conversion of Th17 cells. STAT3 blockade efficiently abolished the 5-HT-associated conversion of Th17 cells from Tregs. In summary, patients with AR exhibited higher serum concentrations of 5-HT. 5-HT-primed DCs could convert Tregs to Th17 cells.
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