Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure
Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure
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泰国和委内瑞拉狗体内三种埃里希体的混合感染，重点考虑 16S 核糖体 DNA 二级结构
DOI：
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发表时间：
2001
影响因子：
9.4
通讯作者：
E. Breitschwerdt
中科院分区：
文献类型：
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作者：
J. Suksawat;C. Pitulle;C. Arraga;Karina Madrigal;S. Hancock;E. Breitschwerdt
作为一项调查来自泰国和委内瑞拉的狗的蜱传感染的大型研究的一部分，记录了来自两个国家的两只狗同时感染三种埃利希体，成为本研究的重点。虽然两只狗都没有可归因于埃利希体病的临床症状，但两只狗都是贫血和中性粒细胞减少，泰国狗是血小板减少。针对16S rRNA基因的属特异性和种特异性PCR结果表明，两只狗同时感染了犬埃利希体、鸭状伊氏体和马伊氏伊氏体。据我们所知，这些结果为e的存在提供了第一个分子文件。这些国家的狗都是马。使用通用细菌PCR引物，可以从每只狗中扩增出一个几乎全长的16S rRNA基因。结果表明，该序列与鸭嘴兽（AF156784）的序列基本一致，为这两个地理位置不同的国家首次报道鸭嘴兽16S核糖体DNA （rDNA）序列。为了确定这些序列差异是否允许这两个菌株与其他已发表的16S rDNA E. platys序列区分，我们对rRNA进行了系统发育分析，并考虑了二级结构。
ABSTRACT As part of a larger study to investigate tick-borne infections in dogs from Thailand and Venezuela, documentation of coinfection with three Ehrlichia species in two dogs, one from each country, became the focus of the present study. Although neither dog had clinical signs attributable to ehrlichiosis, both dogs were anemic and neutropenic and the Thai dog was thrombocytopenic. Genus- and species-specific PCR targeting the 16S rRNA genes indicated that both dogs were coinfected with Ehrlichia canis, E. platys, and E. equi. To our knowledge, these results provide the first molecular documentation for the presence ofE. equi in dogs from these countries. Using universal bacterial PCR primers, one nearly full-length 16S rRNA gene could be amplified from each dog. The sequences were identical to each other and almost identical to that of E. platys (AF156784 ), providing the first E. platys 16S ribosomal DNA (rDNA) sequences reported from these two geographically divergent countries. To determine whether these sequence differences allow differentiation between these two strains and other published 16S rDNA E. platys sequences, we performed a phylogenetic analysis of the rRNA, incorporating the consideration of secondary structure.
影响因子：
158.5
作者：
Goodman, JL;Nelson, C;Munderloh, UG
通讯作者：
Munderloh, UG