Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
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DOI：
10.1007/978-1-62703-751-8_16
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Lewis, John D.
中科院分区：
文献类型：
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作者：
Cho, Choi-Fong;Shukla, Sourabh;Lewis, John D.
病毒纳米颗粒（VNPs）是一类新型的生物纳米材料，利用病毒的天然生物相容性用于开发治疗剂，疫苗和成像工具。植物病毒，豇豆花叶病毒（CPMV），已成功地工程化，创造新的癌症靶向成像剂，通过纳入荧光染料，聚乙二醇（PEG）聚合物，和靶向部分。使用简单的缀合策略，可以合成对癌症特异性分子靶标具有高选择性的VNPs，用于肿瘤的体内成像。在这里，我们描述了基于CPMV的VNPs的合成和纯化，使用点击化学对这些VNPs进行功能化，以及它们在动物模型中用于异种移植肿瘤成像的用途。用荧光染料、PEG和靶向配体修饰的VNPs可以在一天内合成，成像研究可以在数小时、数天或数周内进行，这取决于应用。
Viral nanoparticles (VNPs) are a novel class of bionanomaterials that harness the natural biocompatibility of viruses for the development of therapeutics, vaccines, and imaging tools. The plant virus, cowpea mosaic virus (CPMV), has been successfully engineered to create novel cancer-targeted imaging agents by incorporating fluorescent dyes, polyethylene glycol (PEG) polymers, and targeting moieties. Using straightforward conjugation strategies, VNPs with high selectivity for cancer-specific molecular targets can be synthesized for in vivo imaging of tumors. Here we describe the synthesis and purification of CPMV-based VNPs, the functionalization of these VNPs using click chemistry, and their use for imaging xenograft tumors in animal models. VNPs decorated with fluorescent dyes, PEG, and targeting ligands can be synthesized in one day, and imaging studies can be performed over hours, days, or weeks, depending on the application.