THE CELL-SURFACE OF MOUSE DENDRITIC CELLS - FACS ANALYSES OF DENDRITIC CELLS FROM DIFFERENT TISSUES INCLUDING THYMUS
THE CELL-SURFACE OF MOUSE DENDRITIC CELLS - FACS ANALYSES OF DENDRITIC CELLS FROM DIFFERENT TISSUES INCLUDING THYMUS
复制标题
DOI：
10.1016/0008-8749(89)90361-4
复制
发表时间：
1989-01-01
影响因子：
4.3
通讯作者：
STEINMAN, RM
中科院分区：
文献类型：
--
作者：
CROWLEY, M;INABA, K;STEINMAN, RM
来自小鼠脾脏、胸腺和表皮的树突状细胞的表面已与一组单克隆抗体和流式细胞术进行了比较。首先开发了一种方法来分离纯度> 90%的大的贴壁胸腺树突细胞群。这些通过胶原酶消化释放，并在过夜培养后与粘附的巨噬细胞分离。富集基于以下事实：大多数巨噬细胞保持塑料粘附，并与抗体包被的红细胞强烈玫瑰花状。与脾脏一样，胸腺树突状细胞呈星状，具有丰富的I类和II类MHC产物，缺乏许多标准的巨噬细胞和淋巴细胞标志物，并积极刺激混合白细胞反应。大多数脾和胸腺树突状细胞可以被7 D4 mAb裂解，裂解为低亲和力IL-2受体和补体，但通过FACS检测的7 D4水平较低，有时不高于背景。用其他mAb观察到来自不同组织的树突状细胞之间的差异。贴壁树突状细胞均表达J11 d“B细胞”抗原和NL 145交错突细胞标志物，但缺乏33 D1脾树突状细胞抗原。80%至90%的脾树突状细胞为J11 d-、NL 145-、33 D1+，但其余的表达J11 d+、NL 145+、33 D1-胸腺表型。后者的表型也与表皮朗格汉斯细胞的表型相同。我们假设，主要的33 D1+细胞在脾脏中代表了一个迁移阶段，树突状细胞从组织转移到淋巴器官。
The surface of dendritic cells from mouse spleen, thymus, and epidermis has been compared with a panel of monoclonal antibodies and the FACS. A method was first developed to isolate populations of large, adherent, thymic dendritic cells that were > 90% pure. These were released by collagenase digestion and separated from adherent macrophages after overnight culture. Enrichment was based on the facts that most macrophages remained plastic adherent and rosetted strongly with antibody-coated erythrocytes. As in spleen, thymic dendritic cells were stellate in shape, had abundant class I and II MHC products, lacked many standard macrophage and lymphocyte markers, and actively stimulated the mixed leukocyte reaction. Most spleen and thymic dendritic cells could be lysed by the 7D4 mAb, to the low-affinity IL-2 receptor, and complement but the levels of 7D4 by FACS were low and sometimes not above background. Differences among dendritic cells from different tissues were noted with other mAb. Adherent dendritic cells from thymus all expressed the J11d "B cell" antigen and the NL145 interdigitating cell marker, but lacked the 33D1 spleen dendritic cell antigen. Eighty to ninety percent of spleen dendritic cells were J11d-, NL145-, 33D1+ but the remainder expressed the J11d+, NL145+, 33D1- thymic phenotype. The latter phenotype also was identical to that of epidermal Langerhans'' cells. We postulate that the major 33D1+ cell in spleen represents a migratory stage in which dendritic cells are moving from tissues to lymphoid organs.