Interaction of a nuclear factor with the polyomavirus enhancer region.
Interaction of a nuclear factor with the polyomavirus enhancer region.
复制标题
核因子与多瘤病毒增强子区域的相互作用。
DOI：
10.1073/pnas.83.22.8550
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Hearing,P
中科院分区：
文献类型：
--
作者：
Ostapchuk,P;Diffley,JF;Bruder,JT;Stillman,B;Levine,AJ;Hearing,P
我们已经确定了一个因子存在于未分化的F9小鼠胚胎癌细胞的核提取物，特异性地与多瘤病毒增强子区域相互作用。使用核酸酶“足迹”分析来定义精确对应于Veldman等人定义的多瘤增强子元件C的边界的结合位点[Veldman，G. M.，卢普顿，S. & Kamen，R.（1985）Mol. Cell. 5，649-658]，其作为有效病毒DNA复制和早期和晚期区域转录所需的增强子。核酸酶保护区含有一个6碱基对的反向重复序列，由3个碱基对分开，和对称的侧翼DNA酶I超敏切割位点，表明该因子可能作为二聚体结合。克隆的29个碱基对多瘤DNA片段包含完整的结合结构域。在从分化的鼠F9细胞以及鼠L细胞和人HeLa细胞制备的核提取物中发现类似水平的结合活性。该因子已被称为“EF-C”，用于多瘤因子C的增强子结合因子。
We have identified a factor present in nuclear extracts of undifferentiated F9 murine embryonal carcinoma cells that specifically interacts with the polyomavirus enhancer region. Nuclease "footprint" analysis was used to define the binding site that corresponds precisely to the boundaries of polyoma enhancer element C defined by Veldman et al. [Veldman, G. M., Lupton, S. & Kamen, R. (1985) Mol. Cell. Biol. 5, 649-658] that is required as an enhancer for efficient viral DNA replication and early and late region transcription. The region of nuclease protection contains a 6-base-pair inverted repeat, separated by 3 base pairs, and symmetrical flanking DNase I hypersensitive cleavage sites, suggesting that this factor may bind as a dimer. A cloned 29-base-pair polyoma DNA fragment contains an intact binding domain. Similar levels of binding activity were found in nuclear extracts prepared from differentiated murine F9 cells, as well as murine L cells and human HeLa cells. The factor has been termed "EF-C" for enhancer binding factor to polyoma element C.