Novel approach to the detection and quantification of phenolic compounds in olive oil based on 31P nuclear magnetic resonance spectroscopy
Novel approach to the detection and quantification of phenolic compounds in olive oil based on 31P nuclear magnetic resonance spectroscopy
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DOI：
10.1021/jf058138u
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发表时间：
2006-02-08
影响因子：
6.1
通讯作者：
Dais, P
中科院分区：
文献类型：
--
作者：
Christophoridou, S;Dais, P
P-31核磁共振光谱已被用来检测和定量的酚类化合物的极性馏分初榨橄榄油。这种新的分析方法是基于衍生化的羟基和羧基的酚类化合物与2-氯-4，4，5，5-四甲基二氧磷杂环戊烷和鉴定的磷酰化的化合物的基础上的P-31化学位移。通过整合P-31 NMR光谱中的适当信号和使用亚磷酸化环己醇作为内标物，可以实现对初榨橄榄油中大量酚类化合物的定量。最后，这种技术的定量测量的有效性进行了彻底检查。
P-31 NMR spectroscopy has been employed to detect and quantify phenolic compounds in the polar fraction of virgin olive oil. This novel analytical method is based on the derivatization of the hydroxyl and carboxyl groups of phenolic compounds with 2-chloro-4,4,5,5-tetramethyldioxaphospholane and the identification of the phosphitylated compounds on the basis of the P-31 chemical shifts. Quantification of a large number of phenolic compounds in virgin olive oil can be accomplished by integration of the appropriate signals in the P-31 NMR spectrum and the use of the phosphitylated cyclohexanol as internal standard. Finally, the validity of this technique for quantitative measurements was thoroughly examined.