Novelty and Novel Objects Increase c-Fos Immunoreactivity in Mossy Cells in the Mouse Dentate Gyrus
Novelty and Novel Objects Increase c-Fos Immunoreactivity in Mossy Cells in the Mouse Dentate Gyrus
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DOI：
10.1155/2019/1815371
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发表时间：
2019-08-27
影响因子：
3.1
通讯作者：
Scharfman, Helen E.
中科院分区：
文献类型：
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作者：
Bernstein, Hannah L.;Lu, Yi-Ling;Scharfman, Helen E.
齿状回（DG）及其主要细胞类型，颗粒细胞（GC），被认为是许多认知功能的关键。DG的主要神经元亚型是门苔藓细胞（MC）。MCs被认为在认知中起着重要作用，但在体内研究，以了解在认知任务中的MCs的活动是具有挑战性的，因为实验通常涉及上覆海马或DG的创伤，杀死门神经元。此外，通常会发生束缚，并且MC活性会因短暂的束缚应激而降低。社会孤立经常发生，并可能造成混淆。因此，我们使用c-fos蛋白表达来了解MCs何时在其家笼中的社会圈养的成年C57 BL/6小鼠中体内活跃。我们专注于c-fos蛋白的表达后，动物探索新的对象，基于以前的工作表明，MCs表达c-fos蛋白很容易响应一个新的住房位置。此外，MC是需要的训练组件的新的对象定位任务和新奇的编码过程中的食物相关的任务。GluR 2/3作为MCs的标志物。结果表明，新刺激后MC c-fos蛋白表达显著增加，尤其是腹侧DG。我们还发现，新的物体比熟悉的物体产生更高的c-fos水平。有趣的是，一小部分不表达GluR 2/3的神经元在接触新物体后也增加了c-fos蛋白。相比之下，GC似乎相对不敏感。这些结果支持了越来越多的赞赏的DG在新奇检测和新的物体识别，其中门神经元，特别是MC是非常敏感的作用。
The dentate gyrus (DG) and its primary cell type, the granule cell (GC), are thought to be critical to many cognitive functions. A major neuronal subtype of the DG is the hilar mossy cell (MC). MCs have been considered to play an important role in cognition, but in vivo studies to understand the activity of MCs during cognitive tasks are challenging because the experiments usually involve trauma to the overlying hippocampus or DG, which kills hilar neurons. In addition, restraint typically occurs, and MC activity is reduced by brief restraint stress. Social isolation often occurs and is potentially confounding. Therefore, we used c-fos protein expression to understand when MCs are active in vivo in socially housed adult C57BL/6 mice in their home cage. We focused on c-fos protein expression after animals explored novel objects, based on previous work which showed that MCs express c-fos protein readily in response to a novel housing location. Also, MCs are required for the training component of the novel object location task and novelty-encoding during a food-related task. GluR2/3 was used as a marker of MCs. The results showed that MC c-fos protein is greatly increased after exposure to novel objects, especially in ventral DG. We also found that novel objects produced higher c-fos levels than familiar objects. Interestingly, a small subset of neurons that did not express GluR2/3 also increased c-fos protein after novel object exposure. In contrast, GCs appeared relatively insensitive. The results support a growing appreciation of the role of the DG in novelty detection and novel object recognition, where hilar neurons and especially MCs are very sensitive.