Cutting edge:: A new tool to evaluate human pre-erythrocytic malaria vaccines:: Rodent parasites bearing a hybrid Plasmodium falciparum circumsporozoite protein
Cutting edge:: A new tool to evaluate human pre-erythrocytic malaria vaccines:: Rodent parasites bearing a hybrid Plasmodium falciparum circumsporozoite protein
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DOI：
10.4049/jimmunol.169.12.6681
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发表时间：
2002-12-15
影响因子：
4.4
通讯作者：
Nardin, E
中科院分区：
文献类型：
--
作者：
Persson, C;Oliveira, GA;Nardin, E
含有恶性疟原虫环子孢子蛋白重复结构域的疟疾疫苗正在进行人体试验。没有简单的方法来评估疫苗诱导的应答对恶性疟原虫子孢子感染性的影响。与啮齿类疟疾伯氏疟原虫不同，恶性疟原虫子孢子不感染普通实验室动物，仅在体外人类肝细胞培养物中发育。我们产生了携带恶性疟原虫环子孢子蛋白重复序列的重组伯氏疟原虫。这些杂交子孢子在体内和体外都是完全感染性的。针对恶性疟原虫重复序列的单克隆和多克隆Ab中和杂交寄生虫感染性，并且用恶性疟原虫疫苗免疫的小鼠被保护免受杂交子孢子的攻击。
Malaria vaccines containing the Plasmodium falciparum Circumsporozoite protein repeat domain are undergoing human trials. There is no simple method to evaluate the effect of vaccine-induced responses on P. falciparum sporozoite infectivity. Unlike the rodent malaria Plasmodium berghei, P. falciparum sporozoites do not infect common laboratory animals and only develop in vitro in human hepatocyte cultures. We generated a recombinant P. berghei parasite bearing P. falciparum Circumsporozoite protein repeats. These hybrid sporozoites are fully infective in vivo and in vitro. Monoclonal and polyclonal Abs to P. falciparum repeats neutralize hybrid parasite infectivity, and mice immunized with a P. falciparum vaccine are protected against challenge with hybrid sporozoites.