Healing of focal injury in the rat liver.
Healing of focal injury in the rat liver.
复制标题
大鼠肝脏局部损伤的愈合。
作者：
K. Ogawa;J. Suzuki;M. Narasaki;M. Mori
用光镜、电镜和放射自显影技术观察了液氮冷针和火针在大鼠肝内造成的局灶性肝损伤的愈合过程。损伤后24小时，坏死区域与周围正常肝组织明显分界，其边缘被单核细胞、巨噬细胞、中性粒细胞和血小板浸润。炎症浸润后，Ito细胞、胆管细胞和毛细血管内皮细胞在坏死的周边区域增殖。随着Ito细胞的增殖，胶原纤维明显增加。放射自显影显示坏死区增殖的细胞主要是Ito细胞，邻近损伤部位的肝细胞显示DNA合成。坏死和纤维化区域的大小逐渐减小;到2周时，受损部位恢复到几乎正常的组织学外观，偶尔残留瘢痕。这项研究的结果表明，小的局灶性肝损伤局部修复的机制类似于伤口愈合，伊藤细胞在这一过程中发挥了重要作用。
The healing process of focal hepatic injury produced by thrusting a liquid nitrogen-cooled or fire-heated needle in rat liver was investigated by light and electron microscopy and autoradiography. Twenty-four hours after the injury, necrotic areas were sharply demarcated from the surrounding normal hepatic tissue, and their margin was infiltrated with monocytes, macrophages, neutrophils, and platelets. Following the inflammatory infiltration, Ito cells, cholangiolar cells, and capillary endothelial cells proliferated in the peripheral areas of the necrosis. In accordance with the proliferation of Ito cells, collagen fibers markedly increased. Autoradiography demonstrated that cells proliferating in necrotic areas were predominantly Ito cells, and that hepatocytes adjacent to the damaged sites showed DNA synthesis. Necrotic and fibrotic areas were gradually decreased in size; and by 2 weeks, damaged sites were restored to almost normal histologic appearance, occasional scars remaining. The results of this study indicate that small focal hepatic injury is repaired locally by a mechanism resembling that of wound healing, and that Ito cells play an important role in that process.