ROLE OF TRIGLYCERIDE FATTY-ACID CYCLE IN CONTROLLING FAT-METABOLISM IN HUMANS DURING AND AFTER EXERCISE
ROLE OF TRIGLYCERIDE FATTY-ACID CYCLE IN CONTROLLING FAT-METABOLISM IN HUMANS DURING AND AFTER EXERCISE
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DOI：
10.1152/ajpendo.1990.258.2.e382
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发表时间：
1990-02-01
影响因子：
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通讯作者：
WEBER, JM
中科院分区：
文献类型：
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作者：
WOLFE, RR;KLEIN, S;WEBER, JM
我们研究了甘油三酯-脂肪酸循环在增强对运动响应和运动恢复的脂肪酸净通量控制中的作用。 5 名正常志愿者在休息期间、4 小时最大耗氧量 40% 的跑步机锻炼以及 2 小时恢复期间注射 [1-13C] 棕榈酸酯和 D-5-甘油。通过间接量热法量化总脂肪氧化。脂肪分解（甘油的出现率）从 2.1.+- 增加。 0.3 至 6.0.+-. 1.2.mu.mol.cntdot.kg-1.cntdot。运动 30 分钟后 min-1，此后逐渐增加至 10.5 .+- 值。 4小时后0.8.mu.mol.cntdot.kg-1.cntdot.min-1。恢复前20分钟内脂肪分解迅速下降，但恢复2小时后仍显着升高。游离脂肪酸的出现率遵循相同的反应模式。 70% 的释放脂肪酸在休息时被重新酯化，而在运动的前 30 分钟内，该值下降至 25%。再酯化保持<脂肪分解的35%，直到开始恢复，此时该值上升至90%。在运动中，超过一半的脂肪氧化增加可归因于再酯化百分比的减少。运动和恢复期间再酯化百分比的大部分变化是由释放到血浆中的脂肪酸的细胞外循环的变化引起的。我们得出的结论是，甘油三酯-脂肪酸循环在使脂肪酸代谢对能量代谢的重大变化做出快速响应方面发挥着重要作用。
We have investigated the role of triglyceride-fatty acid cycling in amplifying control of the net flux of fatty acids in response to exercise and in recovery from exercise. Five normal volunteers were infused with [1-13C]palmitate and D-5-glycerol throughout rest, 4 h of treadmill excercise at 40% maximum O2 consumption, and 2 h of recovery. Total fat oxidation was quantified by indirect calorimetry. Lipolysis (rate of appearance of glycerol) increased from 2.1 .+-. 0.3 to 6.0 .+-. 1.2 .mu.mol.cntdot.kg-1 .cntdot. min-1 after 30 min of exercise and progressively increased thereafter to a value of 10.5 .+-. 0.8 .mu.mol.cntdot.kg-1.cntdot.min-1 after 4 h. Lipolysis decreased rapidly during the first 20 min of recovery, but it was still significantly elevated after 2 h of recovery. The rate of appearance of free fatty acids followed the same pattern of response. Seventy percent of released fatty acids were reesterified at rest, and this value decreased to 25% within the first 30 min of exercise. Reesterification remained < 35% of lipolysis until the start of recovery, at which time the value rose to 90%. In exercise, more than one-half the increase in fat oxidation could be attributed to the reduction in the percent reesterification. Most of the change in percent reesterification during exercise and recovery was caused by changes in extracellular cycling of fatty acids released into plasma. We conclude that triglyceride-fatty acid cycling plays an important role in enabling a rapid response of fatty acid metabolism to major changes in energy metabolism.