Gingival Crevicular Fluid and Serum Visfatin Concentration: Their Relationship in Periodontal Health and Disease
Gingival Crevicular Fluid and Serum Visfatin Concentration: Their Relationship in Periodontal Health and Disease
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DOI：
10.1902/jop.2011.100690
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发表时间：
2011-09-01
影响因子：
4.3
通讯作者：
Sharma, Anuj
中科院分区：
文献类型：
--
作者：
Pradeep, A. R.;Raghavendra, N. M.;Sharma, Anuj
背景资料：内脂素是一种多效性介质，作为生长因子、细胞因子和参与能量代谢（包括烟酰胺腺嘌呤二核苷酸代谢）的酶，最近被证明发挥多种促炎功能。本研究的目的是确定内脂素在龈沟液（GCF）和血清样本中的存在，并找出它们之间的关联，如果有的话。（20名男性和20名女性;年龄范围：23 ~ 53岁），根据牙龈指数、探诊深度、临床附着水平，和放射学参数（骨丢失）。组1（10名牙周组织健康的患者;年龄范围：25-36岁），组2（15例牙龈炎患者;年龄范围：25-36岁），第3组（15例慢性牙周炎患者;年龄范围：23-53岁）（通过微毛细管移液管）和血清（通过静脉穿刺）收集样本以使用酶联免疫吸附测定试剂盒估计内脂素水平。从健康组到牙龈炎组再到牙周炎组，龈沟液和血清中内脂素浓度均随疾病严重程度的增加而增加，差异有显著性（P
Background: Visfatin is a pleiotropic mediator that acts as growth factor, cytokine, and enzyme involved in energy including nicotinamide adenine dinucleotide metabolism and was recently demonstrated to exert several proinflammatory functions. The purpose of this study is to determine the presence of visfatin in gingival crevicular fluid (GCF) and serum samples and to find out their association, if any.Methods: At the beginning of the study, 40 individuals (20 males and 20 females; age range: 23 to 53 years) were selected and divided into three groups based on the gingival index, probing depths, clinical attachment levels, and radiologic parameters (bone loss). Group 1 (10 patients with healthy periodontium; age range: 25-36 years), group 2 (15 patients with gingivitis; age range: 25-36 years), and group 3 (15 patients with chronic periodontitis; age range: 23-53 years) GCF (by microcapillary pipettes) and serum(by venipuncture) samples were collected to estimate levels of visfatin using an enzyme-linked immunosorbent assay kit.Results: Mean visfatin concentrations increased in GCF and serum with the severity of disease from healthy to gingivitis to periodontitis groups and differed significantly (P