Retinoic acid signaling is essential for pancreas development and promotes endocrine at the expense of exocrine cell differentiation in Xenopus
Retinoic acid signaling is essential for pancreas development and promotes endocrine at the expense of exocrine cell differentiation in Xenopus
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DOI：
10.1016/j.ydbio.2004.03.030
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Pieler, T
中科院分区：
文献类型：
--
作者：
Chen, YL;Pan, FC;Pieler, T
脊椎动物内胚层如何以及何时首先细分为离散的祖细胞群，这些祖细胞群将产生不同的主要器官，包括胰腺和肝脏，人们对此知之甚少。我们使用非洲爪蟾作为模型系统来表征这些事件，因为它特别适合研究脊椎动物的早期胚胎模式。我们的实验结果支持这样的观点，即视黄酸（RA）在非洲爪蟾中起着重要的内胚层模式信号的作用，并且它早在原肠胚形成期间就发挥作用。 RA治疗的结果是，Sonic Hedgehog (Shh)（一种已知的其他脊椎动物系统中胰腺发育的抑制剂）的表达在背侧胰前内胚层中受到负向调节。此外，还发现 RA 以牺牲背侧胰腺的外分泌分化为代价来促进内分泌，这与该区域中 Notch 信号活动的特异性抑制相关。相反，RA 增强腹侧胰腺外分泌标记基因的表达。 (C) 2004 Elsevier Inc. 保留所有权利。
How and when the vertebrate endoderm is first subdivided into discrete progenitor cell populations that will give rise to the different major organs, including pancreas and liver, are only poorly understood. We have used Xenopus laevis as a model system to characterize these events, since it is particularly suited to study the early embryonic patterning in vertebrates. Our experimental results support the notion that retinoic acid (RA) functions as an essential endodermal patterning signal in Xenopus and that it acts as early as during gastrulation. As a result of RA treatment, the expression of Sonic Hedgehog (Shh), a known inhibitor of pancreas development in other vertebrate systems, is negatively regulated in the dorsal prepancreatic endoderm. Furthermore, RA is found to promote endocrine at the expense of exocrine differentiation in the dorsal pancreas, correlating with a specific inhibition of Notch signaling activities in this territory. Conversely, RA enhances exocrine marker gene expression in the ventral pancreas. (C) 2004 Elsevier Inc. All rights reserved.